Analysis of Crude Protein and Allergen Abundance in Peanuts (Arachis hypogaea cv. Walter) from Three Growing Regions in Australia

Nicole E. Walczyk, Penelope M C Smith, Euan Tovey, Graeme C. Wright, Dayle B. Fleischfresser, Thomas H. Roberts*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effects of plant growth conditions on concentrations of proteins, including allergens, in peanut (Arachis hypogaea L.) kernels are largely unknown. Peanuts (cv. Walter) were grown at five sites (Taabinga, Redvale, Childers, Bundaberg, and Kairi) covering three commercial growing regions in Queensland, Australia. Differences in temperature, rainfall, and solar radiation during the growing season were evaluated. Kernel yield varied from 2.3 t/ha (Kairi) to 3.9 t/ha (Childers), probably due to differences in solar radiation. Crude protein appeared to vary only between Kairi and Childers, whereas Ara h 1 and 2 concentrations were similar in all locations. 2D-DIGE revealed significant differences in spot volumes for only two minor protein spots from peanuts grown in the five locations. Western blotting using peanut-allergic serum revealed no qualitative differences in recognition of antigens. It was concluded that peanuts grown in different growing regions in Queensland, Australia, had similar protein compositions and therefore were unlikely to show differences in allergenicity.

Original languageEnglish
Pages (from-to)3714-3725
Number of pages12
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number15
DOIs
Publication statusPublished - 17 Apr 2013

Keywords

  • 2D-DIGE
  • allergen
  • allergenicity
  • Ara h 1
  • Ara h 2
  • Arachis hypogaea
  • growth conditions
  • peanut

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