Analysis of human leukaemias and lymphomas using extensive immunophenotypes from an antibody microarray

Larissa Belov, Stephen P. Mulligan, Nicole Barber, Adrian Woolfson, Mike Scott, Kerryn Stoner, Jeremy S. Chrisp, William A. Sewell, Kenneth F. Bradstock, Linda Bendall, Dana S. Pascovici, Mervyn Thomas, Wendy Erber, Pauline Huang, Mary Sartor, Graham A. R. Young, James S. Wiley, Surender Juneja, William G. Wierda, Anthony R. GreenMichael J. Keating, Richard I. Christopherson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

A novel antibody microarray has been developed that provides an extensive immunophenotype of leukaemia cells. The assay is a solid phase cell-capture technique in which 82 antigens are studied simultaneously. This paper presents the analysis of 733 patients with a variety of leukaemias and lymphomas from peripheral blood and bone marrow. Discriminant Function Analysis of the expression profiles from these 733 patients and 63 normal subjects were clustered and showed high levels of consistency with diagnoses obtained using conventional clinical and laboratory criteria. The overall levels of consensus for classification using the microarray compared with established criteria were 93.9% (495/527 patients) for peripheral blood and 97.6% (201/206 patients) for bone marrow aspirates, showing that the extensive phenotype alone was frequently able to classify the disease when the leukaemic clone was the dominant cell population present. Immunophenotypes for neoplastic cells were distinguishable from normal cells when the leukaemic cell count was at least 5 × 109 cells/l in peripheral blood, or 20% of cells obtained from bone marrow aspirates. This technique may be a useful adjunct to flow cytometry and other methods when an extensive phenotype of the leukaemia cell is desired for clinical trials, research and prognostic factor analysis.

Original languageEnglish
Pages (from-to)184-197
Number of pages14
JournalBritish Journal of Haematology
Volume135
Issue number2
DOIs
Publication statusPublished - Oct 2006
Externally publishedYes

Keywords

  • leukaemia
  • CD antigens
  • immunophenotype
  • expression profile
  • array

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