Analysis of lipid A from Salmonella minnesota R595 lipopolysaccharide by chemical methods and nuclear magnetic resonance

M. Batley*, P. A. McNicholas, N. H. Packer, J. W. Redmond

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Modifications were introduced in established chemical methods in an attempt to develop reliable means of analysis of lipid A from Salmonella minnesota R595 lipopolysaccharide. Modified hydrolysis conditions led to an estimate of glucosamine content that was significantly higher than established values. This estimate is consistent with the amount of amide-linked fatty acid determined from kinetic studies of fatty acid release and with the presently accepted number of 3-deoxy-D-mannooctulosonic acid residues. Quantitative nuclear magnetic resonance spectroscopy was used to provide independent absolute estimates of the phosphorus-to-glucosamine ratio and of the level of total phosphate and fatty acids; these estimates were consistent with those obtained from chemical methods.

Original languageEnglish
Pages (from-to)449-451
Number of pages3
JournalReviews of Infectious Diseases
Volume6
Issue number4
Publication statusPublished - Jul 1984

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