Analysis of microalgae lipid production using high-throughput fluorescence imaging flow cytometry

M. Li, H. E. Muñoz, J. Lin, K. Owsley, E. Diebold, K. Goda, D. Di Carlo*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

Microalgae such as Euglena gracilis (E. gracilis) are capable of energy-rich lipid production, and have been shown to be a promising source of biofuel. Identifying and selecting E. gracilis strains with higher lipid production per biomass at a single cell level can increase the organism's value for biofuel generation. We propose a high-throughput method to identify E. gracilis cells with varied levels of lipid yield via fluorescence imaging flow cytometry based on radiofrequency-tagged emission (FIRE). By utilizing this imaging method and image post-processing techniques, we are able to extract useful information, such as individual shape metrics, and lipid production per cell.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Place of PublicationSan Diego, California
PublisherChemical and Biological Microsystems Society
Pages307-308
Number of pages2
ISBN (Print)9781510834163
Publication statusPublished - 2016
Externally publishedYes
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period9/10/1613/10/16

Keywords

  • Biofuel
  • Image processing
  • Microalgae
  • Fluorescence imaging flow cytometry

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  • Cite this

    Li, M., Muñoz, H. E., Lin, J., Owsley, K., Diebold, E., Goda, K., & Di Carlo, D. (2016). Analysis of microalgae lipid production using high-throughput fluorescence imaging flow cytometry. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) (pp. 307-308). San Diego, California: Chemical and Biological Microsystems Society.