Analysis of microalgae lipid production using high-throughput fluorescence imaging flow cytometry

M. Li; H. E. Muñoz; J. Lin; K. Owsley; E. Diebold; K. Goda; D. Di Carlo

Analysis of microalgae lipid production using high-throughput fluorescence imaging flow cytometry

M. Li, H. E. Muñoz, J. Lin, K. Owsley, E. Diebold, K. Goda, D. Di Carlo^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution

Abstract

Microalgae such as Euglena gracilis (E. gracilis) are capable of energy-rich lipid production, and have been shown to be a promising source of biofuel. Identifying and selecting E. gracilis strains with higher lipid production per biomass at a single cell level can increase the organism's value for biofuel generation. We propose a high-throughput method to identify E. gracilis cells with varied levels of lipid yield via fluorescence imaging flow cytometry based on radiofrequency-tagged emission (FIRE). By utilizing this imaging method and image post-processing techniques, we are able to extract useful information, such as individual shape metrics, and lipid production per cell.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Place of Publication	San Diego, California
Publisher	Chemical and Biological Microsystems Society
Pages	307-308
Number of pages	2
ISBN (Print)	9781510834163
Publication status	Published - 2016
Externally published	Yes
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	9/10/16 → 13/10/16

Keywords

Biofuel
Image processing
Microalgae
Fluorescence imaging flow cytometry

Cite this

@inproceedings{76da6fca597b4a6094588f0b7f4fc860,

title = "Analysis of microalgae lipid production using high-throughput fluorescence imaging flow cytometry",

abstract = "Microalgae such as Euglena gracilis (E. gracilis) are capable of energy-rich lipid production, and have been shown to be a promising source of biofuel. Identifying and selecting E. gracilis strains with higher lipid production per biomass at a single cell level can increase the organism's value for biofuel generation. We propose a high-throughput method to identify E. gracilis cells with varied levels of lipid yield via fluorescence imaging flow cytometry based on radiofrequency-tagged emission (FIRE). By utilizing this imaging method and image post-processing techniques, we are able to extract useful information, such as individual shape metrics, and lipid production per cell.",

keywords = "Biofuel, Image processing, Microalgae, Fluorescence imaging flow cytometry",

author = "M. Li and Mu{\~n}oz, {H. E.} and J. Lin and K. Owsley and E. Diebold and K. Goda and {Di Carlo}, D.",

year = "2016",

language = "English",

isbn = "9781510834163",

pages = "307--308",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)",

publisher = "Chemical and Biological Microsystems Society",

note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

}

Li, M, Muñoz, HE, Lin, J, Owsley, K, Diebold, E, Goda, K & Di Carlo, D 2016, Analysis of microalgae lipid production using high-throughput fluorescence imaging flow cytometry. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). Chemical and Biological Microsystems Society, San Diego, California, pp. 307-308, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 9/10/16.

Analysis of microalgae lipid production using high-throughput fluorescence imaging flow cytometry. / Li, M.; Muñoz, H. E.; Lin, J. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, California: Chemical and Biological Microsystems Society, 2016. p. 307-308.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution

TY - GEN

T1 - Analysis of microalgae lipid production using high-throughput fluorescence imaging flow cytometry

AU - Li, M.

AU - Muñoz, H. E.

AU - Lin, J.

AU - Owsley, K.

AU - Diebold, E.

AU - Goda, K.

AU - Di Carlo, D.

PY - 2016

Y1 - 2016

N2 - Microalgae such as Euglena gracilis (E. gracilis) are capable of energy-rich lipid production, and have been shown to be a promising source of biofuel. Identifying and selecting E. gracilis strains with higher lipid production per biomass at a single cell level can increase the organism's value for biofuel generation. We propose a high-throughput method to identify E. gracilis cells with varied levels of lipid yield via fluorescence imaging flow cytometry based on radiofrequency-tagged emission (FIRE). By utilizing this imaging method and image post-processing techniques, we are able to extract useful information, such as individual shape metrics, and lipid production per cell.

AB - Microalgae such as Euglena gracilis (E. gracilis) are capable of energy-rich lipid production, and have been shown to be a promising source of biofuel. Identifying and selecting E. gracilis strains with higher lipid production per biomass at a single cell level can increase the organism's value for biofuel generation. We propose a high-throughput method to identify E. gracilis cells with varied levels of lipid yield via fluorescence imaging flow cytometry based on radiofrequency-tagged emission (FIRE). By utilizing this imaging method and image post-processing techniques, we are able to extract useful information, such as individual shape metrics, and lipid production per cell.

KW - Biofuel

KW - Image processing

KW - Microalgae

KW - Fluorescence imaging flow cytometry

UR - http://www.scopus.com/inward/record.url?scp=85014181047&partnerID=8YFLogxK

M3 - Conference proceeding contribution

AN - SCOPUS:85014181047

SN - 9781510834163

SP - 307

EP - 308

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

PB - Chemical and Biological Microsystems Society

CY - San Diego, California

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Y2 - 9 October 2016 through 13 October 2016

ER -

Analysis of microalgae lipid production using high-throughput fluorescence imaging flow cytometry

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Keywords

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