@inbook{75a88acdee8a4e248b8463f368089cad,
title = "Analysis of rice proteins using SDS-PAGE shotgun proteomics",
abstract = "In this chapter we describe the workflow used in our laboratory to analyze rice leaf samples using label-free shotgun proteomics based on SDS-PAGE fractionation of proteins. Rice proteomics has benefitted substantially from successful execution of shotgun proteomics techniques. We describe steps on how to proceed starting from rice protein extraction, SDS-PAGE, in-gel protein digestion with trypsin, nanoLC-MS/MS, and database searching using the GPM. Data from these experiments can be used for spectral counting, where simultaneous quantitation of several thousand proteins can be obtained.",
keywords = "Label-free, Plant proteomics, Rice, SDS-PAGE, Shotgun proteomics",
author = "Neilson, {Karlie A.} and George, {Iniga S.} and Emery, {Samantha J.} and Sridevi Muralidharan and Mehdi Mirzaei and Haynes, {Paul A.}",
year = "2014",
doi = "10.1007/978-1-62703-631-3_21",
language = "English",
isbn = "9781627036306",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "289--302",
editor = "Jorrin-Novo, {Jesus V.} and Setsuko Komatsu and Wolfram Weckwerth and Stefanie Wienkoop",
booktitle = "Plant proteomics",
edition = "2",
}