Anandamide is a partial agonist at native vanilloid receptors in acutely isolated mouse trigeminal sensory neurons

Louise A. Roberts, MacDonald J. Christie, Mark Connor

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

1 The endogenous fatty acid anandamide (AEA) is a partial agonist at cannabinoid CB1 receptors and has been reported to be a full agonist at the recombinant vanilloid receptor, VR1. 2 Whole cell voltage clamp techniques were used to examine the efficacy of AEA and related analogues methanandamide and N-(4-hydroxyphenyl)-arachidonylamide (AM404) at native VR1 receptors in acutely isolated mouse trigeminal neurons. 3 Superfusion of the VR1 agonist capsaicin onto small trigeminal neurons voltage clamped at + 40 mV produced outward currents in most cells, with a pEC50 of 6.3 ± 0.1 (maximum currents at 10-30 μM). 4 AEA produced outward currents with a pEC50 of 5.6 ± 0.1. Maximal AEA currents (30-100 μM) were 38 ± 2% of the capsaicin maximum. AEA currents were blocked by the VR1 antagonist capsazepine (30 μM), but unaffected by the CB1 antagonist SR141716A (1 μM). Methanandamide and AM404 were less potent than AEA at activating VR1. 5 Methanandamide (100 μM) produced currents 37=6% of the capsaicin maximum, the highest concentration of AM404 tested (100 μM) produced currents that were 55 ± 9% of the capsaicin maximum. 6 Capsazepine abolished the currents produced by AM404 (100 μM) and strongly attenuated (>70%) those produced by methanandamide (100 μM). 7 Co-superfusion of AEA (30 μM, methanandamide (100 μM) or AM404 (100 μM) with capsaicin (3 μM) resulted in a significant reduction of the capsaicin current. 8 These data indicate that AEA, methanandamide and AM404 activate native VR1 receptors, but that all three compounds are partial agonists when compared with capsaicin.

Original languageEnglish
Pages (from-to)421-428
Number of pages8
JournalBritish Journal of Pharmacology
Volume137
Issue number4
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • AM404
  • Capsaicin
  • Efficacy
  • Methanandamide
  • Nociception
  • VRI

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