Angiotensin II Receptor Expression Following Intestinal Transplantation in Mice

Emma Spak*, Sara Ewert, Michael Vieth, Lars Fändriks, Anders Åneman, Michael Olausson, George Dindelegan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Background: To further improve the success rate of intestinal transplantation there is a need to find early appearing indicators of rejection. The specific aim of this study was to compare Angiotensin (Ang) II type 1 receptor and Ang II type 2 receptor expression in relation to histological signs of rejection. Methods: Mice of the C57BL6 strain with syngeneic intestinal grafts were compared to mice subjected to allogeneic intestinal transplantation with BalbC strain as donors. Local expression of Ang II type 1 and 2 receptor was evaluated using rt-PCR and Western blot and compared to histological picture in grafts and native intestine. Results: The Ang II type 2 receptor protein expression was markedly up-regulated in the allogeneically transplanted graft from day 1 postoperatively. Histological signs of rejection were not seen until day 6. Conclusion: Intestinal allograft transplantation in mice is associated with a marked up-regulation of the Ang II type 2 receptor. However, the detailed role of the renin-angiotensin system in the immune rejection following intestinal transplantation remains to be clarified.

Original languageEnglish
Pages (from-to)144-149
Number of pages6
JournalJournal of Surgical Research
Volume135
Issue number1
DOIs
Publication statusPublished - Sep 2006

Keywords

  • angiotensin II receptors
  • intestinal transplantation
  • renin-angiotensin system

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