Antibacterial activity in the sea urchin, Heliocidaris erythrogramma

G. S. Wilson, D. A. Raftos, S. V. Nair, T. Roberts

Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution

Abstract

The sea urchin, Heliocidaris erythrogramma, has potent antibacterial activity in its coelomic fluid. Antibacterial activity was detected by a colorimetric bactericidal assay, which revealed a minimum inhibitory concentration of 680 ppm coelomic fluid v/v in FSW. The antibacterial activity of a number of molecules was induced by bacterial challenge with maximal antibacterial activity occurring after 12 h. The molecules responsible for antibacterial activity were heat-stable and also induced by non-specific challenge. Polar extracts of coelomic fluid indicated that at least one of the factors contributing to antibacterial activity was proteinaceous. The antimicrobial peptide was approximately 13 9 kDa as determined by SDS-PAGE. The amino acid sequence and potency of the antimicrobial peptide is also discussed.
Original languageEnglish
Title of host publication31st Lorne proteins conference, 2006
Place of PublicationParkville, Vic
PublisherLorne Proteins Organising Committee
Pages68
Number of pages1
ISBN (Print)9781604237504
Publication statusPublished - 2006
EventLorne conference on protein structure and function (31st : 2006) - Lorne, Vic.
Duration: 5 Feb 20069 Feb 2006

Conference

ConferenceLorne conference on protein structure and function (31st : 2006)
CityLorne, Vic.
Period5/02/069/02/06

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