Abstract
The sea urchin, Heliocidaris erythrogramma, has potent antibacterial activity in its coelomic fluid. Antibacterial activity was detected by a colorimetric bactericidal assay, which revealed a minimum inhibitory concentration of 680 ppm coelomic fluid v/v in FSW. The antibacterial activity of a number of molecules was induced by bacterial challenge with maximal antibacterial activity occurring after 12 h. The molecules responsible for antibacterial activity were heat-stable and also induced by non-specific challenge.
Polar extracts of coelomic fluid indicated that at least one of the factors contributing to antibacterial activity was proteinaceous. The antimicrobial peptide was approximately 13 9 kDa as determined by SDS-PAGE. The amino acid sequence and potency of the antimicrobial peptide is also discussed.
| Original language | English |
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| Title of host publication | 31st Lorne proteins conference, 2006 |
| Place of Publication | Parkville, Vic |
| Publisher | Lorne Proteins Organising Committee |
| Pages | 68 |
| Number of pages | 1 |
| ISBN (Print) | 9781604237504 |
| Publication status | Published - 2006 |
| Event | Lorne conference on protein structure and function (31st : 2006) - Lorne, Vic. Duration: 5 Feb 2006 → 9 Feb 2006 |
Conference
| Conference | Lorne conference on protein structure and function (31st : 2006) |
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| City | Lorne, Vic. |
| Period | 5/02/06 → 9/02/06 |