Antimicrobial mode of action of secretions from the metapleural gland of Myrmecia gulosa (Australian bull ant)

J. A. Mackintosh, J. E. Trimble, M. K. Jones, P. H. Karuso, A. J. Beattie, D. A. Veal

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Secretions from exocrine metapleural glands of Myrmecia gulosa (Australian bull ant) exhibit broad-spectrum antimicrobial activity. Treatment of the yeast Candida albicans with metapleural secretion resulted in the rapid and total leakage of K± ions from cells within 10 min. Ultrastructural analysis of the bacteria Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa, and cells and protoplasts of Candida albicans demonstrated gross damage of the cell membrane and aggregation of the cytoplasmic matrix of treated cells. Degradation of membrane-bound organelles was also observed in Candida albicans. The antimicrobially active components of metapleural secretions were nonpolar and interacted with the phospholipid bilayer, causing damage to the structural integrity of liposomes and the release of carboxyfluorescein. The data suggest that the antimicrobial agents in metapleural secretion act primarily by disrupting the structure and function of the phospholipid bilayer of the cytoplasmic membrane.

LanguageEnglish
Pages136-144
Number of pages9
JournalCanadian Journal of Microbiology
Volume41
Issue number2
Publication statusPublished - 1995

Fingerprint

Ants
Candida
Candida albicans
Phospholipids
Cell Membrane
Exocrine Glands
Bacillus cereus
Membranes
Cell Aggregation
Protoplasts
Structural integrity
Cell membranes
Anti-Infective Agents
Liposomes
Organelles
Yeast
Pseudomonas aeruginosa
Escherichia coli
Bacteria
Agglomeration

Keywords

  • ant metapleural secretion
  • antimicrobial
  • Candida albicans
  • cytoplasmic membrane

Cite this

@article{71cf1fb7d2ef4b94ac1b4a6f69268d2a,
title = "Antimicrobial mode of action of secretions from the metapleural gland of Myrmecia gulosa (Australian bull ant)",
abstract = "Secretions from exocrine metapleural glands of Myrmecia gulosa (Australian bull ant) exhibit broad-spectrum antimicrobial activity. Treatment of the yeast Candida albicans with metapleural secretion resulted in the rapid and total leakage of K± ions from cells within 10 min. Ultrastructural analysis of the bacteria Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa, and cells and protoplasts of Candida albicans demonstrated gross damage of the cell membrane and aggregation of the cytoplasmic matrix of treated cells. Degradation of membrane-bound organelles was also observed in Candida albicans. The antimicrobially active components of metapleural secretions were nonpolar and interacted with the phospholipid bilayer, causing damage to the structural integrity of liposomes and the release of carboxyfluorescein. The data suggest that the antimicrobial agents in metapleural secretion act primarily by disrupting the structure and function of the phospholipid bilayer of the cytoplasmic membrane.",
keywords = "ant metapleural secretion, antimicrobial, Candida albicans, cytoplasmic membrane",
author = "Mackintosh, {J. A.} and Trimble, {J. E.} and Jones, {M. K.} and Karuso, {P. H.} and Beattie, {A. J.} and Veal, {D. A.}",
year = "1995",
language = "English",
volume = "41",
pages = "136--144",
journal = "Canadian Journal of Microbiology",
issn = "0008-4166",
publisher = "National Research Council of Canada",
number = "2",

}

Antimicrobial mode of action of secretions from the metapleural gland of Myrmecia gulosa (Australian bull ant). / Mackintosh, J. A.; Trimble, J. E.; Jones, M. K.; Karuso, P. H.; Beattie, A. J.; Veal, D. A.

In: Canadian Journal of Microbiology, Vol. 41, No. 2, 1995, p. 136-144.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antimicrobial mode of action of secretions from the metapleural gland of Myrmecia gulosa (Australian bull ant)

AU - Mackintosh, J. A.

AU - Trimble, J. E.

AU - Jones, M. K.

AU - Karuso, P. H.

AU - Beattie, A. J.

AU - Veal, D. A.

PY - 1995

Y1 - 1995

N2 - Secretions from exocrine metapleural glands of Myrmecia gulosa (Australian bull ant) exhibit broad-spectrum antimicrobial activity. Treatment of the yeast Candida albicans with metapleural secretion resulted in the rapid and total leakage of K± ions from cells within 10 min. Ultrastructural analysis of the bacteria Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa, and cells and protoplasts of Candida albicans demonstrated gross damage of the cell membrane and aggregation of the cytoplasmic matrix of treated cells. Degradation of membrane-bound organelles was also observed in Candida albicans. The antimicrobially active components of metapleural secretions were nonpolar and interacted with the phospholipid bilayer, causing damage to the structural integrity of liposomes and the release of carboxyfluorescein. The data suggest that the antimicrobial agents in metapleural secretion act primarily by disrupting the structure and function of the phospholipid bilayer of the cytoplasmic membrane.

AB - Secretions from exocrine metapleural glands of Myrmecia gulosa (Australian bull ant) exhibit broad-spectrum antimicrobial activity. Treatment of the yeast Candida albicans with metapleural secretion resulted in the rapid and total leakage of K± ions from cells within 10 min. Ultrastructural analysis of the bacteria Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa, and cells and protoplasts of Candida albicans demonstrated gross damage of the cell membrane and aggregation of the cytoplasmic matrix of treated cells. Degradation of membrane-bound organelles was also observed in Candida albicans. The antimicrobially active components of metapleural secretions were nonpolar and interacted with the phospholipid bilayer, causing damage to the structural integrity of liposomes and the release of carboxyfluorescein. The data suggest that the antimicrobial agents in metapleural secretion act primarily by disrupting the structure and function of the phospholipid bilayer of the cytoplasmic membrane.

KW - ant metapleural secretion

KW - antimicrobial

KW - Candida albicans

KW - cytoplasmic membrane

UR - http://www.scopus.com/inward/record.url?scp=0028944003&partnerID=8YFLogxK

M3 - Article

VL - 41

SP - 136

EP - 144

JO - Canadian Journal of Microbiology

T2 - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

SN - 0008-4166

IS - 2

ER -