Antioxidant efficacy and the upregulation of Nrf2-mediated HO-1 expression by (+)-lariciresinol, a lignan isolated from Rubia philippinensis, through the activation of p38

Vivek K. Bajpai, Md Badrul Alam, Khong Trong Quan, Kyoo-Ri Kwon, Mi-Kyoung Ju, Hee-Jeong Choi, Jong Sung Lee, Jung-In Yoon, Rajib Majumder, Irfan A. Rather, Kangmin Kim, Sang-Han Lee, MinKyun Na

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The aim of the present study was to examine the antioxidative activity of (+)-lariciresinol (LRSL), an optically active lignan isolated from Rubia philippinensis in several in vitro assays. LRSL was also subjected to evaluate its inhibitory effect against the generation of reactive oxygen species (ROS) in murine macrophage (RAW 264.7) cells. The results showed that LRSL possessed very strong radical scavenging activity and reducing power, as well as inhibited ROS generation in a dose-dependent manner without showing any cytotoxicity. The transcriptional and translational levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were markedly higher in the sample treated group. LRSL treatment also increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) with a corresponding increase in the transcriptional and translational activities of the heme oxygenase-1 (HO-1). LRSL activated p38 and treatments with SB239063 (a p38 inhibitor) suppressed the LRSL-induced activation of Nrf2, resulting in a decrease in HO-1 expression. Collectively, the data demonstrated that LRSL has potent antioxidative activity, decreasing ROS generation in RAW 264.7 cells and increasing the transcriptional and translational levels of antioxidant enzymes by activating Nrf2-mediated HO-1 induction via p38 signaling.

LanguageEnglish
Article number46035
Pages1-10
Number of pages10
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 5 Apr 2017

Fingerprint

heme oxygenase (biliverdin-producing)
Rubia
lignans
reactive oxygen species
antioxidants
antioxidant activity
glutathione peroxidase
cytotoxicity
catalase
superoxide dismutase
macrophages
cells
mice
assays
dosage
enzymes
sampling

Bibliographical note

Copyright the Author(s) 2017. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Cite this

Bajpai, Vivek K. ; Alam, Md Badrul ; Quan, Khong Trong ; Kwon, Kyoo-Ri ; Ju, Mi-Kyoung ; Choi, Hee-Jeong ; Lee, Jong Sung ; Yoon, Jung-In ; Majumder, Rajib ; Rather, Irfan A. ; Kim, Kangmin ; Lee, Sang-Han ; Na, MinKyun. / Antioxidant efficacy and the upregulation of Nrf2-mediated HO-1 expression by (+)-lariciresinol, a lignan isolated from Rubia philippinensis, through the activation of p38. In: Scientific Reports. 2017 ; Vol. 7. pp. 1-10.
@article{c1e68337a2fe42f68da30838a76468e8,
title = "Antioxidant efficacy and the upregulation of Nrf2-mediated HO-1 expression by (+)-lariciresinol, a lignan isolated from Rubia philippinensis, through the activation of p38",
abstract = "The aim of the present study was to examine the antioxidative activity of (+)-lariciresinol (LRSL), an optically active lignan isolated from Rubia philippinensis in several in vitro assays. LRSL was also subjected to evaluate its inhibitory effect against the generation of reactive oxygen species (ROS) in murine macrophage (RAW 264.7) cells. The results showed that LRSL possessed very strong radical scavenging activity and reducing power, as well as inhibited ROS generation in a dose-dependent manner without showing any cytotoxicity. The transcriptional and translational levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were markedly higher in the sample treated group. LRSL treatment also increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) with a corresponding increase in the transcriptional and translational activities of the heme oxygenase-1 (HO-1). LRSL activated p38 and treatments with SB239063 (a p38 inhibitor) suppressed the LRSL-induced activation of Nrf2, resulting in a decrease in HO-1 expression. Collectively, the data demonstrated that LRSL has potent antioxidative activity, decreasing ROS generation in RAW 264.7 cells and increasing the transcriptional and translational levels of antioxidant enzymes by activating Nrf2-mediated HO-1 induction via p38 signaling.",
author = "Bajpai, {Vivek K.} and Alam, {Md Badrul} and Quan, {Khong Trong} and Kyoo-Ri Kwon and Mi-Kyoung Ju and Hee-Jeong Choi and Lee, {Jong Sung} and Jung-In Yoon and Rajib Majumder and Rather, {Irfan A.} and Kangmin Kim and Sang-Han Lee and MinKyun Na",
note = "Copyright the Author(s) 2017. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",
year = "2017",
month = "4",
day = "5",
doi = "10.1038/srep46035",
language = "English",
volume = "7",
pages = "1--10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Springer, Springer Nature",

}

Bajpai, VK, Alam, MB, Quan, KT, Kwon, K-R, Ju, M-K, Choi, H-J, Lee, JS, Yoon, J-I, Majumder, R, Rather, IA, Kim, K, Lee, S-H & Na, M 2017, 'Antioxidant efficacy and the upregulation of Nrf2-mediated HO-1 expression by (+)-lariciresinol, a lignan isolated from Rubia philippinensis, through the activation of p38', Scientific Reports, vol. 7, 46035, pp. 1-10. https://doi.org/10.1038/srep46035

Antioxidant efficacy and the upregulation of Nrf2-mediated HO-1 expression by (+)-lariciresinol, a lignan isolated from Rubia philippinensis, through the activation of p38. / Bajpai, Vivek K.; Alam, Md Badrul; Quan, Khong Trong; Kwon, Kyoo-Ri; Ju, Mi-Kyoung; Choi, Hee-Jeong; Lee, Jong Sung; Yoon, Jung-In; Majumder, Rajib; Rather, Irfan A.; Kim, Kangmin; Lee, Sang-Han; Na, MinKyun.

In: Scientific Reports, Vol. 7, 46035, 05.04.2017, p. 1-10.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antioxidant efficacy and the upregulation of Nrf2-mediated HO-1 expression by (+)-lariciresinol, a lignan isolated from Rubia philippinensis, through the activation of p38

AU - Bajpai, Vivek K.

AU - Alam, Md Badrul

AU - Quan, Khong Trong

AU - Kwon, Kyoo-Ri

AU - Ju, Mi-Kyoung

AU - Choi, Hee-Jeong

AU - Lee, Jong Sung

AU - Yoon, Jung-In

AU - Majumder, Rajib

AU - Rather, Irfan A.

AU - Kim, Kangmin

AU - Lee, Sang-Han

AU - Na, MinKyun

N1 - Copyright the Author(s) 2017. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2017/4/5

Y1 - 2017/4/5

N2 - The aim of the present study was to examine the antioxidative activity of (+)-lariciresinol (LRSL), an optically active lignan isolated from Rubia philippinensis in several in vitro assays. LRSL was also subjected to evaluate its inhibitory effect against the generation of reactive oxygen species (ROS) in murine macrophage (RAW 264.7) cells. The results showed that LRSL possessed very strong radical scavenging activity and reducing power, as well as inhibited ROS generation in a dose-dependent manner without showing any cytotoxicity. The transcriptional and translational levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were markedly higher in the sample treated group. LRSL treatment also increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) with a corresponding increase in the transcriptional and translational activities of the heme oxygenase-1 (HO-1). LRSL activated p38 and treatments with SB239063 (a p38 inhibitor) suppressed the LRSL-induced activation of Nrf2, resulting in a decrease in HO-1 expression. Collectively, the data demonstrated that LRSL has potent antioxidative activity, decreasing ROS generation in RAW 264.7 cells and increasing the transcriptional and translational levels of antioxidant enzymes by activating Nrf2-mediated HO-1 induction via p38 signaling.

AB - The aim of the present study was to examine the antioxidative activity of (+)-lariciresinol (LRSL), an optically active lignan isolated from Rubia philippinensis in several in vitro assays. LRSL was also subjected to evaluate its inhibitory effect against the generation of reactive oxygen species (ROS) in murine macrophage (RAW 264.7) cells. The results showed that LRSL possessed very strong radical scavenging activity and reducing power, as well as inhibited ROS generation in a dose-dependent manner without showing any cytotoxicity. The transcriptional and translational levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were markedly higher in the sample treated group. LRSL treatment also increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) with a corresponding increase in the transcriptional and translational activities of the heme oxygenase-1 (HO-1). LRSL activated p38 and treatments with SB239063 (a p38 inhibitor) suppressed the LRSL-induced activation of Nrf2, resulting in a decrease in HO-1 expression. Collectively, the data demonstrated that LRSL has potent antioxidative activity, decreasing ROS generation in RAW 264.7 cells and increasing the transcriptional and translational levels of antioxidant enzymes by activating Nrf2-mediated HO-1 induction via p38 signaling.

UR - http://www.scopus.com/inward/record.url?scp=85017181184&partnerID=8YFLogxK

U2 - 10.1038/srep46035

DO - 10.1038/srep46035

M3 - Article

VL - 7

SP - 1

EP - 10

JO - Scientific Reports

T2 - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 46035

ER -