ANTISENSE OLIGONUCLEOTIDES

A NEW TOOL IN NEUROSCIENCE

Paul M. Pilowsky*, Satoshi Suzuki, Jane B. Minson

*Corresponding author for this work

    Research output: Contribution to journalReview article

    34 Citations (Scopus)

    Abstract

    1. Recent studies have shown that gene expression can be selectively attenuated by administration of short sequences of nucleotides (oligonucleotides) that are complementary to a portion of messenger RNA coding for a particular gene product. 2. This technique is known as ANTISENSE, because the oligonucleotides are complementary to the mRNA which has the same sequence as the SENSE strand of DNA. 3. In the present review we focus, after a brief discussion of gene expression and mechanisms of action of ANTISENSE, on the methodological aspects of ANTISENSE experiments in neuroscience. In particular, we address the advantages, disadvantages and controls for the ANTISENSE technique, as well as the choice, design, mode of delivery, dose and storage of ANTISENSE oligonucleotides.

    Original languageEnglish
    Pages (from-to)935-944
    Number of pages10
    JournalClinical and Experimental Pharmacology and Physiology
    Volume21
    Issue number12
    DOIs
    Publication statusPublished - 1994

    Keywords

    • antisense
    • Fos
    • gene
    • nervous system
    • oligonucleotides
    • phosphorothioate.

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