Antitumor effects of Auraptene in 4T1 tumor-bearing Balb/c mice

Mohammad Reza Shiran, Davar Amani, Abolghasem Ajami, Mahshad Jalalpourroodsari, Maghsoud Khalizadeh, Mohsen Rashidi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Objectives: Breast cancer is a common malignant tumor in women with limited treatment options and multiple side effects. Today, the anti-cancer properties of natural compounds have attracted widespread attention from researchers worldwide.

Methods: In this study, we treated 4T1 tumor-bearing Balb/c mice with intraperitoneal injection of Auraptene, paraffin oil, and saline as two control groups. Body weight and tumor volume were measured before and after treatment. Hematoxylin and eosin (H & E) staining and immunohistochemistry of Ki-67 were used as markers of proliferation. In addition, ELISA assays were performed to assess serum IFN-γand IL-4 levels.

Results: There was no significant change in body weight in all animal groups before and after treatment. 10 days after the last treatment, Auraptene showed its anti-cancer effect, which was confirmed by the smaller tumor volume and H & E staining. In addition, Ki-67 expression levels were significantly reduced in tumor samples from the Auraptene-treated group compared to the paraffin oil and saline-treated groups. In addition, in tumor-bearing and normal mice receiving Auraptene treatment, IL-4 serum production levels were reduced, while serum levels of IFN-γwere significantly up-regulated in tumor-bearing mice after Auraptene treatment.

Conclusions: In the case of inhibition of tumor volume and Ki-67 proliferation markers, Auraptene can effectively inhibit tumor growth in breast cancer animal models. In addition, it might increases Th1 and CD8 + T cell responses after reducing IL-4 serum levels and IFN-γupregulation, respectively. However, further research is needed to clarify its mechanism of action.

Original languageEnglish
Pages (from-to)245-252
Number of pages8
JournalHormone Molecular Biology and Clinical Investigation
Volume42
Issue number3
DOIs
Publication statusPublished - Sept 2021

Keywords

  • angiogenesis
  • anti-angiogenesis
  • Auraptene
  • breast cancer
  • immunostimulatory activity
  • natural compounds

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