TY - JOUR
T1 - Antitumor effects of Auraptene in 4T1 tumor-bearing Balb/c mice
AU - Shiran, Mohammad Reza
AU - Amani, Davar
AU - Ajami, Abolghasem
AU - Jalalpourroodsari, Mahshad
AU - Khalizadeh, Maghsoud
AU - Rashidi, Mohsen
PY - 2021/9
Y1 - 2021/9
N2 - Objectives: Breast cancer is a common malignant tumor in women with limited treatment options and multiple side effects. Today, the anti-cancer properties of natural compounds have attracted widespread attention from researchers worldwide.Methods: In this study, we treated 4T1 tumor-bearing Balb/c mice with intraperitoneal injection of Auraptene, paraffin oil, and saline as two control groups. Body weight and tumor volume were measured before and after treatment. Hematoxylin and eosin (H & E) staining and immunohistochemistry of Ki-67 were used as markers of proliferation. In addition, ELISA assays were performed to assess serum IFN-γand IL-4 levels.Results: There was no significant change in body weight in all animal groups before and after treatment. 10 days after the last treatment, Auraptene showed its anti-cancer effect, which was confirmed by the smaller tumor volume and H & E staining. In addition, Ki-67 expression levels were significantly reduced in tumor samples from the Auraptene-treated group compared to the paraffin oil and saline-treated groups. In addition, in tumor-bearing and normal mice receiving Auraptene treatment, IL-4 serum production levels were reduced, while serum levels of IFN-γwere significantly up-regulated in tumor-bearing mice after Auraptene treatment.Conclusions: In the case of inhibition of tumor volume and Ki-67 proliferation markers, Auraptene can effectively inhibit tumor growth in breast cancer animal models. In addition, it might increases Th1 and CD8 + T cell responses after reducing IL-4 serum levels and IFN-γupregulation, respectively. However, further research is needed to clarify its mechanism of action.
AB - Objectives: Breast cancer is a common malignant tumor in women with limited treatment options and multiple side effects. Today, the anti-cancer properties of natural compounds have attracted widespread attention from researchers worldwide.Methods: In this study, we treated 4T1 tumor-bearing Balb/c mice with intraperitoneal injection of Auraptene, paraffin oil, and saline as two control groups. Body weight and tumor volume were measured before and after treatment. Hematoxylin and eosin (H & E) staining and immunohistochemistry of Ki-67 were used as markers of proliferation. In addition, ELISA assays were performed to assess serum IFN-γand IL-4 levels.Results: There was no significant change in body weight in all animal groups before and after treatment. 10 days after the last treatment, Auraptene showed its anti-cancer effect, which was confirmed by the smaller tumor volume and H & E staining. In addition, Ki-67 expression levels were significantly reduced in tumor samples from the Auraptene-treated group compared to the paraffin oil and saline-treated groups. In addition, in tumor-bearing and normal mice receiving Auraptene treatment, IL-4 serum production levels were reduced, while serum levels of IFN-γwere significantly up-regulated in tumor-bearing mice after Auraptene treatment.Conclusions: In the case of inhibition of tumor volume and Ki-67 proliferation markers, Auraptene can effectively inhibit tumor growth in breast cancer animal models. In addition, it might increases Th1 and CD8 + T cell responses after reducing IL-4 serum levels and IFN-γupregulation, respectively. However, further research is needed to clarify its mechanism of action.
KW - angiogenesis
KW - anti-angiogenesis
KW - Auraptene
KW - breast cancer
KW - immunostimulatory activity
KW - natural compounds
UR - http://www.scopus.com/inward/record.url?scp=85102242810&partnerID=8YFLogxK
U2 - 10.1515/hmbci-2020-0090
DO - 10.1515/hmbci-2020-0090
M3 - Article
C2 - 33675195
AN - SCOPUS:85102242810
SN - 1868-1883
VL - 42
SP - 245
EP - 252
JO - Hormone Molecular Biology and Clinical Investigation
JF - Hormone Molecular Biology and Clinical Investigation
IS - 3
ER -