TY - JOUR
T1 - APC senses cell-cell contacts and moves to the nucleus upon their disruption
AU - Brocardo, Mariana G.
AU - Bianchini, Michele
AU - Radrizzani, Martín
AU - Reyes, Gloria B.
AU - Dugour, Andrea V.
AU - Taminelli, Guillermo L.
AU - Gonzalez Solveyra, César
AU - Santa-Coloma, Tomás A.
PY - 2001
Y1 - 2001
N2 - The adenomatous polyposis coli (ARC) tumor suppressor protein is involved in the Wnt/wingless pathway, modulating β-catenin activity. We report the development of a highly specific, chemically synthesized oligobody (oligonucleotide-based synthetic antibody), directed against the N-terminal region of APC. Using this reagent, we found that within 16 h of disrupting HT-29 cell-cell contacts by harvesting cells with trypsin/EDTA treatment and replating, Arc was translocated from the cytoplasm to the nucleus. Five days after plating the cells, when the cells had returned to their normal confluent phenotype and cell-cell contacts were reestablished, APC returned to the cytoplasm. These results suggest that Arc functions as part of a "sensor" system, and responds to the loss of cell-cell contacts by moving to the nucleus, and returning to the cytoplasm when the contacts are fully restored.
AB - The adenomatous polyposis coli (ARC) tumor suppressor protein is involved in the Wnt/wingless pathway, modulating β-catenin activity. We report the development of a highly specific, chemically synthesized oligobody (oligonucleotide-based synthetic antibody), directed against the N-terminal region of APC. Using this reagent, we found that within 16 h of disrupting HT-29 cell-cell contacts by harvesting cells with trypsin/EDTA treatment and replating, Arc was translocated from the cytoplasm to the nucleus. Five days after plating the cells, when the cells had returned to their normal confluent phenotype and cell-cell contacts were reestablished, APC returned to the cytoplasm. These results suggest that Arc functions as part of a "sensor" system, and responds to the loss of cell-cell contacts by moving to the nucleus, and returning to the cytoplasm when the contacts are fully restored.
KW - APC (adenomatous polyposis coli)
KW - Cell-cell contacts
KW - HT29 cells
KW - Oligobodies, synthetic antibodies
UR - http://www.scopus.com/inward/record.url?scp=0034805354&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2001.5066
DO - 10.1006/bbrc.2001.5066
M3 - Article
C2 - 11409891
AN - SCOPUS:0034805354
SN - 0006-291X
VL - 284
SP - 982
EP - 986
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -