APC senses cell-cell contacts and moves to the nucleus upon their disruption

Mariana G. Brocardo, Michele Bianchini, Martín Radrizzani, Gloria B. Reyes, Andrea V. Dugour, Guillermo L. Taminelli, César Gonzalez Solveyra, Tomás A. Santa-Coloma

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The adenomatous polyposis coli (ARC) tumor suppressor protein is involved in the Wnt/wingless pathway, modulating β-catenin activity. We report the development of a highly specific, chemically synthesized oligobody (oligonucleotide-based synthetic antibody), directed against the N-terminal region of APC. Using this reagent, we found that within 16 h of disrupting HT-29 cell-cell contacts by harvesting cells with trypsin/EDTA treatment and replating, Arc was translocated from the cytoplasm to the nucleus. Five days after plating the cells, when the cells had returned to their normal confluent phenotype and cell-cell contacts were reestablished, APC returned to the cytoplasm. These results suggest that Arc functions as part of a "sensor" system, and responds to the loss of cell-cell contacts by moving to the nucleus, and returning to the cytoplasm when the contacts are fully restored.

LanguageEnglish
Pages982-986
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume284
Issue number4
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Tumor Suppressor Proteins
beta Catenin
Plating
Edetic Acid
Oligonucleotides
Trypsin
Cells
Antibodies
Sensors
Cytoplasm
AIDS-Related Complex
HT29 Cells
Wnt Signaling Pathway
Adenomatous Polyposis Coli
Phenotype

Keywords

  • APC (adenomatous polyposis coli)
  • Cell-cell contacts
  • HT29 cells
  • Oligobodies, synthetic antibodies

Cite this

Brocardo, M. G., Bianchini, M., Radrizzani, M., Reyes, G. B., Dugour, A. V., Taminelli, G. L., ... Santa-Coloma, T. A. (2001). APC senses cell-cell contacts and moves to the nucleus upon their disruption. Biochemical and Biophysical Research Communications, 284(4), 982-986. https://doi.org/10.1006/bbrc.2001.5066
Brocardo, Mariana G. ; Bianchini, Michele ; Radrizzani, Martín ; Reyes, Gloria B. ; Dugour, Andrea V. ; Taminelli, Guillermo L. ; Gonzalez Solveyra, César ; Santa-Coloma, Tomás A. / APC senses cell-cell contacts and moves to the nucleus upon their disruption. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 284, No. 4. pp. 982-986.
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Brocardo, MG, Bianchini, M, Radrizzani, M, Reyes, GB, Dugour, AV, Taminelli, GL, Gonzalez Solveyra, C & Santa-Coloma, TA 2001, 'APC senses cell-cell contacts and moves to the nucleus upon their disruption', Biochemical and Biophysical Research Communications, vol. 284, no. 4, pp. 982-986. https://doi.org/10.1006/bbrc.2001.5066

APC senses cell-cell contacts and moves to the nucleus upon their disruption. / Brocardo, Mariana G.; Bianchini, Michele; Radrizzani, Martín; Reyes, Gloria B.; Dugour, Andrea V.; Taminelli, Guillermo L.; Gonzalez Solveyra, César; Santa-Coloma, Tomás A.

In: Biochemical and Biophysical Research Communications, Vol. 284, No. 4, 2001, p. 982-986.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Dugour, Andrea V.

AU - Taminelli, Guillermo L.

AU - Gonzalez Solveyra, César

AU - Santa-Coloma, Tomás A.

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