Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts

Olivier Laczka; Lucy Skillman; William G. Ditcham; Brenton Hamdorf; Danny K Y Wong; Peter Bergquist; Anwar Sunna

doi:10.1016/j.mimet.2013.08.014

Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts

Olivier Laczka^*, Lucy Skillman, William G. Ditcham, Brenton Hamdorf, Danny K Y Wong, Peter Bergquist, Anwar Sunna

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5×10² Cryptosporidium oocysts per mL in 60min.

Original language	English
Pages (from-to)	182-185
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	95
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2013.08.014
Publication status	Published - Nov 2013

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10.1016/j.mimet.2013.08.014

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abstract = "We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5×102 Cryptosporidium oocysts per mL in 60min.",

author = "Olivier Laczka and Lucy Skillman and Ditcham, {William G.} and Brenton Hamdorf and Wong, {Danny K Y} and Peter Bergquist and Anwar Sunna",

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AU - Laczka, Olivier

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AU - Ditcham, William G.

AU - Hamdorf, Brenton

AU - Wong, Danny K Y

AU - Bergquist, Peter

AU - Sunna, Anwar

PY - 2013/11

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AB - We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5×102 Cryptosporidium oocysts per mL in 60min.

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AN - SCOPUS:84884135198

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ER -

Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts

Abstract

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