Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts

Olivier Laczka, Lucy Skillman, William G. Ditcham, Brenton Hamdorf, Danny K Y Wong, Peter Bergquist, Anwar Sunna

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5×102 Cryptosporidium oocysts per mL in 60min.

LanguageEnglish
Pages182-185
Number of pages4
JournalJournal of Microbiological Methods
Volume95
Issue number2
DOIs
Publication statusPublished - Nov 2013

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Cryptosporidium parvum
Oocysts
Electrodes
Enzyme-Linked Immunosorbent Assay
Cryptosporidium
Antibodies
Horseradish Peroxidase

Cite this

Laczka, Olivier ; Skillman, Lucy ; Ditcham, William G. ; Hamdorf, Brenton ; Wong, Danny K Y ; Bergquist, Peter ; Sunna, Anwar. / Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts. In: Journal of Microbiological Methods. 2013 ; Vol. 95, No. 2. pp. 182-185.
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Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts. / Laczka, Olivier; Skillman, Lucy; Ditcham, William G.; Hamdorf, Brenton; Wong, Danny K Y; Bergquist, Peter; Sunna, Anwar.

In: Journal of Microbiological Methods, Vol. 95, No. 2, 11.2013, p. 182-185.

Research output: Contribution to journalArticleResearchpeer-review

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