Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts

Olivier Laczka*, Lucy Skillman, William G. Ditcham, Brenton Hamdorf, Danny K Y Wong, Peter Bergquist, Anwar Sunna

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5×102 Cryptosporidium oocysts per mL in 60min.

Original languageEnglish
Pages (from-to)182-185
Number of pages4
JournalJournal of Microbiological Methods
Volume95
Issue number2
DOIs
Publication statusPublished - Nov 2013

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