Application of capillary electrophoresis to study phenolic profiles of honeybee-collected pollen

Qingcui Chu, Xiuhui Tian, Lianmei Jiang, Jiannong Ye*

*Corresponding author for this work

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Honeybee-collected pollen is promoted as a health food with a wide range of nutritional and therapeutic properties. A high-performance capillary electrophoresis with amperometric detection method has been developed for the simultaneous determination of bioactive ingredients in 10 samples of honeybee-collected pollen in this work. Under the optimum conditions, 13 phenolic components can be well-separated or nearly baseline-separated (apigenin and vanillic acid peaks) within 29 min at the separation voltage of 14 kV in a 50 mM borax running buffer (pH 9.0), and adequate extraction was obtained with ethanol for the determination of the above 13 compounds. Recovery (94.1-104.0%), repeatability of the peak current (<5.4%), and detection limits (6.9 × 10 -7-6.4 × 10 -9 g mL -1) for the method were evaluated. This procedure was successfully used for the analysis and comparison of the phenolic content of honeybee-collected pollen samples originating from different floral origins based on their electropherograms or "phenolic profiles".

Original languageEnglish
Pages (from-to)8864-8869
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume55
Issue number22
DOIs
Publication statusPublished - 31 Oct 2007
Externally publishedYes

Keywords

  • Amperometric detection
  • Bee pollen
  • Capillary electrophoresis
  • Electromigration profiles
  • Phenolic profiles

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