Abstract
Honeybee-collected pollen is promoted as a health food with a wide range of nutritional and therapeutic properties. A high-performance capillary electrophoresis with amperometric detection method has been developed for the simultaneous determination of bioactive ingredients in 10 samples of honeybee-collected pollen in this work. Under the optimum conditions, 13 phenolic components can be well-separated or nearly baseline-separated (apigenin and vanillic acid peaks) within 29 min at the separation voltage of 14 kV in a 50 mM borax running buffer (pH 9.0), and adequate extraction was obtained with ethanol for the determination of the above 13 compounds. Recovery (94.1-104.0%), repeatability of the peak current (<5.4%), and detection limits (6.9 × 10 -7-6.4 × 10 -9 g mL -1) for the method were evaluated. This procedure was successfully used for the analysis and comparison of the phenolic content of honeybee-collected pollen samples originating from different floral origins based on their electropherograms or "phenolic profiles".
| Original language | English |
|---|---|
| Pages (from-to) | 8864-8869 |
| Number of pages | 6 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 55 |
| Issue number | 22 |
| DOIs | |
| Publication status | Published - 31 Oct 2007 |
| Externally published | Yes |
Keywords
- Amperometric detection
- Bee pollen
- Capillary electrophoresis
- Electromigration profiles
- Phenolic profiles