Application of fermentation for isoflavone extraction from soy molasses

K. C. Duru, E. G. Kovaleva, T. V. Glukhareva

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

4 Citations (Scopus)


Extraction of isoflavones from soy products remains a major challenge for researchers. Different extraction techniques have been employed but the need to use a cheap green extraction technique remains the main focus. This study applied fermentation of soy molasses using Saccharomyces cerevisiae for extraction of isoflavones and compared this technique to the conventional extraction method. The aluminum chloride colorimetric method was used for the determination of total flavonoid content of extracts. The highest yield was observed from extraction using ethyl acetate after fermentation of soy molasses and the lowest one was given by the extract from conventional extraction method. The DPPH radical scavenging activities of the extracts were also compared. The extract obtained using ethyl acetate after fermentation showed the highest antioxidant activity (0.0269 meq), while extract from conventional extraction had the lowest antioxidant activity (0.0055 meq). The effect of time on daidzein yield was studied using HPLC standard addition method. Daidzein concentration was higher in extract obtained at t = 80 min (3.82 ± 0.11 mg of daidzein /g of extract) as compared to that obtained at t = 60 min (2.89 ± 0.10 mg of daidzein /g of extract).
Original languageEnglish
Title of host publicationPhysics, Technologies and Innovation (PTI-2017)
Subtitle of host publicationProceedings of the IV International Young Researchers’ Conference
EditorsAlbina A. Valeeva, Vladimir A. Volkovich
Place of PublicationUnited States
PublisherAmerican Institute of Physics
Number of pages8
ISBN (Electronic)9780735415690
Publication statusPublished - 2017
Externally publishedYes
EventIV International Young Researchers’ Conference: Physics, Technologies and Innovation - Ekaterinburg, Russian Federation
Duration: 15 May 201719 May 2017

Publication series

NameAIP Conference Proceedings
PublisherAmerican Institute of Physics
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616


ConferenceIV International Young Researchers’ Conference
Country/TerritoryRussian Federation


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