Application of the near-field optical microscope to the imaging of non-labelled microtubules and comparison with other techniques

J. D. White; A. Zvyagin; A. Uchida; S. Hisanaga; H. Tatsumi; Y. Katayama; M. Ohtsu

doi:10.1109/CLEOPR.1997.610837

Application of the near-field optical microscope to the imaging of non-labelled microtubules and comparison with other techniques

J. D. White, A. Zvyagin, A. Uchida, S. Hisanaga, H. Tatsumi, Y. Katayama, M. Ohtsu

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

Abstract

Microtubules (MT), formed from the polymerization of tubulin, are important in the cytoskeleton (which maintains cell shape) and in the elongation of the neuronal process. The existence of a number of unanswered questions regarding their assembly/disassembly and resulting dynamic instability is studied. Non-labelled MTs have been observed with a variety of microscopes and the images were compared. Near-field optical microscope (NOM) is seen to offer the advantages of sub-wavelength resolution while retaining the non-intrusive advantages of an optical technique. When coupled with shear force microscopy, NOM uniquely provides a cheap and attractive method of imaging subcellular structures with vertical as well as lateral resolution.

Original language	English
Title of host publication	CLEO/Pacific Rim '97
Subtitle of host publication	Technical Digest CLEO/Pacific Rim '97 Pacific Rim Conference on Lasers and Electro-Optics
Place of Publication	Piscataway, NJ
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Pages	242-243
Number of pages	2
ISBN (Print)	0780338898, 0780338901
DOIs	https://doi.org/10.1109/CLEOPR.1997.610837
Publication status	Published - 1997
Externally published	Yes
Event	CLEO/Pacific Rim 1997: Pacific Rim Conference on Lasers and Electro-Optics - Chiba, Jpn Duration: 14 Jul 1997 → 18 Jul 1997

Other

Other	CLEO/Pacific Rim 1997
City	Chiba, Jpn
Period	14/07/97 → 18/07/97

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10.1109/CLEOPR.1997.610837

Cite this

White, J. D., Zvyagin, A., Uchida, A., Hisanaga, S., Tatsumi, H., Katayama, Y., & Ohtsu, M. (1997). Application of the near-field optical microscope to the imaging of non-labelled microtubules and comparison with other techniques. In CLEO/Pacific Rim '97: Technical Digest CLEO/Pacific Rim '97 Pacific Rim Conference on Lasers and Electro-Optics (pp. 242-243). Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/CLEOPR.1997.610837

White, J. D. ; Zvyagin, A. ; Uchida, A. et al. / Application of the near-field optical microscope to the imaging of non-labelled microtubules and comparison with other techniques. CLEO/Pacific Rim '97: Technical Digest CLEO/Pacific Rim '97 Pacific Rim Conference on Lasers and Electro-Optics. Piscataway, NJ : Institute of Electrical and Electronics Engineers (IEEE), 1997. pp. 242-243

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abstract = "Microtubules (MT), formed from the polymerization of tubulin, are important in the cytoskeleton (which maintains cell shape) and in the elongation of the neuronal process. The existence of a number of unanswered questions regarding their assembly/disassembly and resulting dynamic instability is studied. Non-labelled MTs have been observed with a variety of microscopes and the images were compared. Near-field optical microscope (NOM) is seen to offer the advantages of sub-wavelength resolution while retaining the non-intrusive advantages of an optical technique. When coupled with shear force microscopy, NOM uniquely provides a cheap and attractive method of imaging subcellular structures with vertical as well as lateral resolution.",

author = "White, {J. D.} and A. Zvyagin and A. Uchida and S. Hisanaga and H. Tatsumi and Y. Katayama and M. Ohtsu",

year = "1997",

doi = "10.1109/CLEOPR.1997.610837",

language = "English",

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pages = "242--243",

booktitle = "CLEO/Pacific Rim '97",

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White, JD, Zvyagin, A, Uchida, A, Hisanaga, S, Tatsumi, H, Katayama, Y & Ohtsu, M 1997, Application of the near-field optical microscope to the imaging of non-labelled microtubules and comparison with other techniques. in CLEO/Pacific Rim '97: Technical Digest CLEO/Pacific Rim '97 Pacific Rim Conference on Lasers and Electro-Optics. Institute of Electrical and Electronics Engineers (IEEE), Piscataway, NJ, pp. 242-243, CLEO/Pacific Rim 1997, Chiba, Jpn, 14/07/97. https://doi.org/10.1109/CLEOPR.1997.610837

Application of the near-field optical microscope to the imaging of non-labelled microtubules and comparison with other techniques. / White, J. D.; Zvyagin, A.; Uchida, A. et al.
CLEO/Pacific Rim '97: Technical Digest CLEO/Pacific Rim '97 Pacific Rim Conference on Lasers and Electro-Optics. Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE), 1997. p. 242-243.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

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White JD, Zvyagin A, Uchida A, Hisanaga S, Tatsumi H, Katayama Y et al. Application of the near-field optical microscope to the imaging of non-labelled microtubules and comparison with other techniques. In CLEO/Pacific Rim '97: Technical Digest CLEO/Pacific Rim '97 Pacific Rim Conference on Lasers and Electro-Optics. Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE). 1997. p. 242-243 doi: 10.1109/CLEOPR.1997.610837

Application of the near-field optical microscope to the imaging of non-labelled microtubules and comparison with other techniques

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