Applications of automated amino acid analysis using 9-fluorenylmethyl chloroformate

Paul A. Haynes*, David Sheumack, Lisa G. Greig, Jeffrey Kibby, John W. Redmond

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    79 Citations (Scopus)


    A rapid and sensitive fully automated method for the determination of primary and secondary amino acids in different matrices is described. Amino acids are derivatized with 9-fluorenylmethyl chloroformate using an automated precolumn derivatization technique. Data are presented to show that the technique is both reproducible and highly sensitive. Applications of the technique are presented, including the analysis of peptide and protein hydrolysates and the profiling of free amino acids in physiological fluids.

    Original languageEnglish
    Pages (from-to)107-114
    Number of pages8
    JournalJournal of Chromatography A
    Issue number1-2
    Publication statusPublished - 27 Dec 1991


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