Applications of automated amino acid analysis using 9-fluorenylmethyl chloroformate

Paul A. Haynes; David Sheumack; Lisa G. Greig; Jeffrey Kibby; John W. Redmond

doi:10.1016/0021-9673(91)85012-5

Applications of automated amino acid analysis using 9-fluorenylmethyl chloroformate

Paul A. Haynes^*, David Sheumack, Lisa G. Greig, Jeffrey Kibby, John W. Redmond

^*Corresponding author for this work

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Abstract

A rapid and sensitive fully automated method for the determination of primary and secondary amino acids in different matrices is described. Amino acids are derivatized with 9-fluorenylmethyl chloroformate using an automated precolumn derivatization technique. Data are presented to show that the technique is both reproducible and highly sensitive. Applications of the technique are presented, including the analysis of peptide and protein hydrolysates and the profiling of free amino acids in physiological fluids.

Original language	English
Pages (from-to)	107-114
Number of pages	8
Journal	Journal of Chromatography A
Volume	588
Issue number	1-2
DOIs	https://doi.org/10.1016/0021-9673(91)85012-5
Publication status	Published - 27 Dec 1991

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10.1016/0021-9673(91)85012-5

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abstract = "A rapid and sensitive fully automated method for the determination of primary and secondary amino acids in different matrices is described. Amino acids are derivatized with 9-fluorenylmethyl chloroformate using an automated precolumn derivatization technique. Data are presented to show that the technique is both reproducible and highly sensitive. Applications of the technique are presented, including the analysis of peptide and protein hydrolysates and the profiling of free amino acids in physiological fluids.",

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AU - Haynes, Paul A.

AU - Sheumack, David

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AU - Kibby, Jeffrey

AU - Redmond, John W.

PY - 1991/12/27

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N2 - A rapid and sensitive fully automated method for the determination of primary and secondary amino acids in different matrices is described. Amino acids are derivatized with 9-fluorenylmethyl chloroformate using an automated precolumn derivatization technique. Data are presented to show that the technique is both reproducible and highly sensitive. Applications of the technique are presented, including the analysis of peptide and protein hydrolysates and the profiling of free amino acids in physiological fluids.

AB - A rapid and sensitive fully automated method for the determination of primary and secondary amino acids in different matrices is described. Amino acids are derivatized with 9-fluorenylmethyl chloroformate using an automated precolumn derivatization technique. Data are presented to show that the technique is both reproducible and highly sensitive. Applications of the technique are presented, including the analysis of peptide and protein hydrolysates and the profiling of free amino acids in physiological fluids.

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ER -

Applications of automated amino acid analysis using 9-fluorenylmethyl chloroformate

Abstract

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