Assessing the effect of reactive oxygen species on Escherichia coli using a metabolome approach

Helen Tweeddale, Lucinda Notley-McRobb, Thomas Ferenci*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    41 Citations (Scopus)

    Abstract

    A two-dimensional thin-layer chromatographic analysis of [14C]-labelled metabolites in Escherichia coli was employed to follow metabolic shifts in response to superoxide stress. Steady-state challenge with paraquat at concentrations inducing SoxRS-controlled genes resulted in several alterations in metabolite pools, including a striking increase in valine concentration. Elevated valine levels, together with increased glutathione and alkylperoxidase, are proposed to constitute an intracellular protection mechanism against reactive oxygen species. As shown by this example of metabolome analysis, novel cellular responses to environmental challenge can be revealed by following the total complement of metabolites in a cell.

    Original languageEnglish
    Pages (from-to)237-241
    Number of pages5
    JournalRedox Report
    Volume4
    Issue number5
    Publication statusPublished - 1999

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