Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites

Anahit Penesyan, Francesco Ballestriero, Malak Daim, Staffan Kjelleberg, Torsten Thomas, Suhelen Egan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the "hit-rate" and unmasking multiple activities from the same bacterial source.

Original languageEnglish
Pages (from-to)40-49
Number of pages10
JournalMarine Drugs
Volume11
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Dive into the research topics of 'Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites'. Together they form a unique fingerprint.

Cite this