Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites

Anahit Penesyan; Francesco Ballestriero; Malak Daim; Staffan Kjelleberg; Torsten Thomas; Suhelen Egan

doi:10.3390/md11010040

Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites

Anahit Penesyan, Francesco Ballestriero, Malak Daim, Staffan Kjelleberg, Torsten Thomas, Suhelen Egan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the "hit-rate" and unmasking multiple activities from the same bacterial source.

Original language	English
Pages (from-to)	40-49
Number of pages	10
Journal	Marine Drugs
Volume	11
Issue number	1
DOIs	https://doi.org/10.3390/md11010040
Publication status	Published - Jan 2013

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10.3390/md11010040

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AU - Penesyan, Anahit

AU - Ballestriero, Francesco

AU - Daim, Malak

AU - Kjelleberg, Staffan

AU - Thomas, Torsten

AU - Egan, Suhelen

PY - 2013/1

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AB - A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the "hit-rate" and unmasking multiple activities from the same bacterial source.

UR - https://www.scopus.com/pages/publications/84873118613

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Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites

Abstract

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