ATP-activated cationic and anionic conductances in cultured rat hippocampal neurons

C. Balachandran; M. R. Bennett

doi:10.1016/0304-3940(96)12320-X

ATP-activated cationic and anionic conductances in cultured rat hippocampal neurons

C. Balachandran, M. R. Bennett^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The effects of adenosine 5'-triphosphate (ATP) on voltage-clamped and dissociated rat hippocampal neurons was investigated. Over 30% of neurons possessed ATP-activated inward currents at a holding potential of -70 mV. The ED₅₀ for these currents was 150 μM. At this concentration they were blocked by suramin (1 mM), indicating the involvement of P2-purinoceptors. The P2 purinoceptor agonist potency was 2-methylthio ATP > ATP > ADP > α,β-methylene ATP, thus identifying these purinoceptors as belonging to the P2x subclass. The reversal potential for the ATP-activated currents was -45 ± 8 mV. Ion substitution experiments showed that the permeability ratio for K⁺/Na⁺/Cs⁺/Cl^-, was 18:3:2:1, according to the Goldman-Hodgkin-Katz equation, so that ATP activates cationic and anionic conductances in hippocampal neurons.

Original language	English
Pages (from-to)	73-76
Number of pages	4
Journal	Neuroscience Letters
Volume	204
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(96)12320-X
Publication status	Published - 2 Feb 1996
Externally published	Yes

Keywords

Conductances
Hippocampus
Neurons
Purinoceptors

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10.1016/0304-3940(96)12320-X

Cite this

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title = "ATP-activated cationic and anionic conductances in cultured rat hippocampal neurons",

abstract = "The effects of adenosine 5'-triphosphate (ATP) on voltage-clamped and dissociated rat hippocampal neurons was investigated. Over 30% of neurons possessed ATP-activated inward currents at a holding potential of -70 mV. The ED50 for these currents was 150 μM. At this concentration they were blocked by suramin (1 mM), indicating the involvement of P2-purinoceptors. The P2 purinoceptor agonist potency was 2-methylthio ATP > ATP > ADP > α,β-methylene ATP, thus identifying these purinoceptors as belonging to the P2x subclass. The reversal potential for the ATP-activated currents was -45 ± 8 mV. Ion substitution experiments showed that the permeability ratio for K+/Na+/Cs+/Cl-, was 18:3:2:1, according to the Goldman-Hodgkin-Katz equation, so that ATP activates cationic and anionic conductances in hippocampal neurons.",

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AU - Balachandran, C.

AU - Bennett, M. R.

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N2 - The effects of adenosine 5'-triphosphate (ATP) on voltage-clamped and dissociated rat hippocampal neurons was investigated. Over 30% of neurons possessed ATP-activated inward currents at a holding potential of -70 mV. The ED50 for these currents was 150 μM. At this concentration they were blocked by suramin (1 mM), indicating the involvement of P2-purinoceptors. The P2 purinoceptor agonist potency was 2-methylthio ATP > ATP > ADP > α,β-methylene ATP, thus identifying these purinoceptors as belonging to the P2x subclass. The reversal potential for the ATP-activated currents was -45 ± 8 mV. Ion substitution experiments showed that the permeability ratio for K+/Na+/Cs+/Cl-, was 18:3:2:1, according to the Goldman-Hodgkin-Katz equation, so that ATP activates cationic and anionic conductances in hippocampal neurons.

AB - The effects of adenosine 5'-triphosphate (ATP) on voltage-clamped and dissociated rat hippocampal neurons was investigated. Over 30% of neurons possessed ATP-activated inward currents at a holding potential of -70 mV. The ED50 for these currents was 150 μM. At this concentration they were blocked by suramin (1 mM), indicating the involvement of P2-purinoceptors. The P2 purinoceptor agonist potency was 2-methylthio ATP > ATP > ADP > α,β-methylene ATP, thus identifying these purinoceptors as belonging to the P2x subclass. The reversal potential for the ATP-activated currents was -45 ± 8 mV. Ion substitution experiments showed that the permeability ratio for K+/Na+/Cs+/Cl-, was 18:3:2:1, according to the Goldman-Hodgkin-Katz equation, so that ATP activates cationic and anionic conductances in hippocampal neurons.

KW - Conductances

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KW - Neurons

KW - Purinoceptors

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ATP-activated cationic and anionic conductances in cultured rat hippocampal neurons

Abstract

Keywords

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