Automated locomotor activity monitoring as a quality control assay for mass-reared tephritid flies

Bernard C. Dominiak*, Benjamin G. Fanson, Samuel R. Collins, Phillip W. Taylor

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

BACKGROUND: The Sterile Insect Technique (SIT) requires vast numbers of consistently high quality insects to be produced over long periods. Quality control (QC) procedures are critical to effective SIT, both providing quality assurance and warning of operational deficiencies. We here present a potential new QC assay for mass rearing of Queensland fruit flies (Bactrocera tryoni Froggatt) for SIT; locomotor activity monitoring. We investigated whether automated locomotor activity monitors (LAMs) that simply detect how often a fly passes an infrared sensor in a glass tube might provide similar insights but with much greater economy. RESULTS: Activity levels were generally lower for females than for males, and declined over five days in the monitor for both sexes. Female activity levels were not affected by irradiation, but males irradiated at 60 or 70 Gy had reduced activity levels compared with unirradiated controls. We also found some evidence that mild heat shock of pupae results in adults with reduced activity. CONCLUSION: LAM offers a convenient, effective and economical assay to probe such changes.

Original languageEnglish
Pages (from-to)304-309
Number of pages6
JournalPest Management Science
Volume70
Issue number2
DOIs
Publication statusPublished - Feb 2014

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