TY - JOUR
T1 - Azo dye containing wastewater treatment in earthen membrane based unplanted two chambered constructed wetlands-microbial fuel cells
T2 - a new design for enhanced performance
AU - Mittal, Yamini
AU - Dash, Sudatta
AU - Srivastava, Pratiksha
AU - Mishra, Pravat Manjari
AU - Aminabhavi, Tejraj M.
AU - Yadav, Asheesh Kumar
PY - 2022/1/1
Y1 - 2022/1/1
N2 - This investigation is the first of its kind to enhance detoxification of azo dye and other pollutants containing wastewater using an innovative earthen membrane-based two-chambered constructed wetland cum microbial fuel cell (CW-MFC). The present innovative design simulates the core of a shallow unplanted CW-MFC, which runs the sequential anaerobic and aerobic regimes without mixing of the cathodic and anodic wastewater. The obtained results revealed 94.04 ± 2.87% chemical oxygen demand (COD) and 94.22 ± 1.33% azo dye removal from the synthetic wastewater containing 550 mg/L initial COD and 50 mg/L Methyl orange (MO) azo dye, along with the current density and power density production of 544.6 mA/m3 and 148.29 mW/m3, respectively. The UV-visible spectrum demonstrated azo bond degradation in the anodic region, which was confirmed by the presence of sulphanilic acid as an intermediate of the azo dye degradation in the anodic effluent. The gas chromatography-mass spectrometry (GC-MS) analysis of anodic effluent proved the presence of an another intermediate, N.N-dimethyl-p-phenylenediamine (DMPD) and further confirms mineralization in the cathodic effluent with the elution of several mineralized polar compounds. Phytotoxicity study with Vigna radiata, Triticum aestivum, and Cicer arietinum indicated higher root growth rates (in comparison to control) of 30.72%, 13.53%, and 11.62% in the anodic effluent, whereas 69.70%, 60.28%, and 34.27% in the cathodic effluent, respectively, indicating decreased toxicity. The microbial analysis revealed a shift in microbial community of CW-MFC; the inoculum was abundant with Methanomicrobia class (18.55%), which shifted to class Bacteroidetes (13.99%) in the anodic region which was attributed to azo dye degrading bacteria. Whereas, cathodic microbial community consists of Alpha and Gamma Proteobacteria (59.50%), which are considered as the aromatic ring-degrading microbes.
AB - This investigation is the first of its kind to enhance detoxification of azo dye and other pollutants containing wastewater using an innovative earthen membrane-based two-chambered constructed wetland cum microbial fuel cell (CW-MFC). The present innovative design simulates the core of a shallow unplanted CW-MFC, which runs the sequential anaerobic and aerobic regimes without mixing of the cathodic and anodic wastewater. The obtained results revealed 94.04 ± 2.87% chemical oxygen demand (COD) and 94.22 ± 1.33% azo dye removal from the synthetic wastewater containing 550 mg/L initial COD and 50 mg/L Methyl orange (MO) azo dye, along with the current density and power density production of 544.6 mA/m3 and 148.29 mW/m3, respectively. The UV-visible spectrum demonstrated azo bond degradation in the anodic region, which was confirmed by the presence of sulphanilic acid as an intermediate of the azo dye degradation in the anodic effluent. The gas chromatography-mass spectrometry (GC-MS) analysis of anodic effluent proved the presence of an another intermediate, N.N-dimethyl-p-phenylenediamine (DMPD) and further confirms mineralization in the cathodic effluent with the elution of several mineralized polar compounds. Phytotoxicity study with Vigna radiata, Triticum aestivum, and Cicer arietinum indicated higher root growth rates (in comparison to control) of 30.72%, 13.53%, and 11.62% in the anodic effluent, whereas 69.70%, 60.28%, and 34.27% in the cathodic effluent, respectively, indicating decreased toxicity. The microbial analysis revealed a shift in microbial community of CW-MFC; the inoculum was abundant with Methanomicrobia class (18.55%), which shifted to class Bacteroidetes (13.99%) in the anodic region which was attributed to azo dye degrading bacteria. Whereas, cathodic microbial community consists of Alpha and Gamma Proteobacteria (59.50%), which are considered as the aromatic ring-degrading microbes.
KW - Constructed wetland cum microbial fuel cell
KW - Methyl orange
KW - Chemical oxygen demand
KW - Metagenomic analysis
KW - Phytotoxicity
UR - http://www.scopus.com/inward/record.url?scp=85113808481&partnerID=8YFLogxK
U2 - 10.1016/j.cej.2021.131856
DO - 10.1016/j.cej.2021.131856
M3 - Article
AN - SCOPUS:85113808481
SN - 1385-8947
VL - 427
SP - 1
EP - 12
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
M1 - 131856
ER -