Background-free in-vivo imaging of Vitamin C using time-gateable responsive probe

Bo Song, Zhiqing Ye, Yajie Yang, Hua Ma, Xianlin Zheng, Dayong Jin, Jingli Yuan*

*Corresponding author for this work

Research output: Contribution to journalArticle

28 Citations (Scopus)
12 Downloads (Pure)

Abstract

Sensitive optical imaging of active biomolecules in the living organism requires both a molecular probe specifically responsive to the target and a high-contrast approach to remove the background interference from autofluorescence and light scatterings. Here, a responsive probe for ascorbic acid (vitamin C) has been developed by conjugating two nitroxide radicals with a long-lived luminescent europium complex. The nitroxide radical withholds the probe on its "off" state (barely luminescent), until the presence of vitamin C will switch on the probe by forming its hydroxylamine derivative. The probe showed a linear response to vitamin C concentration with a detection limit of 9.1 nM, two orders of magnitude lower than that achieved using electrochemical methods. Time-gated luminescence microscopy (TGLM) method has further enabled real-time, specific and background-free monitoring of cellular uptake or endogenous production of vitamin C, and mapping of vitamin C in living Daphnia magna. This work suggests a rational design of lanthanide complexes for background-free small animal imaging of biologically functional molecules.

Original languageEnglish
Article number14194
Pages (from-to)1-10
Number of pages10
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 16 Sep 2015

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    ARC CoE Nanoscale BioPhotonics (CNBP) (RAAP)

    Piper, J., Goldys, E., Packer, N. & Jin, D.

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