Barstar:barnase - a versatile platform for colloidal diamond bioconjugation

Varun K A Sreenivasan, Ekaterina A. Ivukina, Wei Deng, Timothy A. Kelf, Tatyana A. Zdobnova, Sergey V. Lukash, Boris V. Veryugin, Oleg A. Stremovskiy, Andrei V. Zvyagin, Sergey M. Deyev

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We report on a bioconjugation platform based on a high-affinity protein pair, barstar:barnase (Bs:Bn), that provides a modular design toolkit capable of strong (covalent) and versatile assembly of bio/nanocomplexes. Luminescent nanodiamonds (140 nm) serve as the docking station to synthesize Bs:Bn bioconjugates which are characterized and utilized in several applications, including cell transfection.

LanguageEnglish
Pages65-68
Number of pages4
JournalJournal of Materials Chemistry
Volume21
Issue number1
DOIs
Publication statusPublished - 7 Jan 2011

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Nanodiamonds
Diamond
Diamonds
Proteins
Bacillus amyloliquefaciens ribonuclease
Bacillus amyloliquefaciens barstar protein

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Sreenivasan, V. K. A., Ivukina, E. A., Deng, W., Kelf, T. A., Zdobnova, T. A., Lukash, S. V., ... Deyev, S. M. (2011). Barstar:barnase - a versatile platform for colloidal diamond bioconjugation. Journal of Materials Chemistry, 21(1), 65-68. https://doi.org/10.1039/c0jm02819c
Sreenivasan, Varun K A ; Ivukina, Ekaterina A. ; Deng, Wei ; Kelf, Timothy A. ; Zdobnova, Tatyana A. ; Lukash, Sergey V. ; Veryugin, Boris V. ; Stremovskiy, Oleg A. ; Zvyagin, Andrei V. ; Deyev, Sergey M. / Barstar:barnase - a versatile platform for colloidal diamond bioconjugation. In: Journal of Materials Chemistry. 2011 ; Vol. 21, No. 1. pp. 65-68.
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Sreenivasan, VKA, Ivukina, EA, Deng, W, Kelf, TA, Zdobnova, TA, Lukash, SV, Veryugin, BV, Stremovskiy, OA, Zvyagin, AV & Deyev, SM 2011, 'Barstar:barnase - a versatile platform for colloidal diamond bioconjugation', Journal of Materials Chemistry, vol. 21, no. 1, pp. 65-68. https://doi.org/10.1039/c0jm02819c

Barstar:barnase - a versatile platform for colloidal diamond bioconjugation. / Sreenivasan, Varun K A; Ivukina, Ekaterina A.; Deng, Wei; Kelf, Timothy A.; Zdobnova, Tatyana A.; Lukash, Sergey V.; Veryugin, Boris V.; Stremovskiy, Oleg A.; Zvyagin, Andrei V.; Deyev, Sergey M.

In: Journal of Materials Chemistry, Vol. 21, No. 1, 07.01.2011, p. 65-68.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Zdobnova, Tatyana A.

AU - Lukash, Sergey V.

AU - Veryugin, Boris V.

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AU - Zvyagin, Andrei V.

AU - Deyev, Sergey M.

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