Barstar:barnase - a versatile platform for colloidal diamond bioconjugation

Varun K A Sreenivasan; Ekaterina A. Ivukina; Wei Deng; Timothy A. Kelf; Tatyana A. Zdobnova; Sergey V. Lukash; Boris V. Veryugin; Oleg A. Stremovskiy; Andrei V. Zvyagin; Sergey M. Deyev

doi:10.1039/c0jm02819c

Barstar:barnase - a versatile platform for colloidal diamond bioconjugation

Varun K A Sreenivasan, Ekaterina A. Ivukina, Wei Deng, Timothy A. Kelf, Tatyana A. Zdobnova, Sergey V. Lukash, Boris V. Veryugin, Oleg A. Stremovskiy, Andrei V. Zvyagin, Sergey M. Deyev

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34 Citations (Scopus)

Abstract

We report on a bioconjugation platform based on a high-affinity protein pair, barstar:barnase (Bs:Bn), that provides a modular design toolkit capable of strong (covalent) and versatile assembly of bio/nanocomplexes. Luminescent nanodiamonds (140 nm) serve as the docking station to synthesize Bs:Bn bioconjugates which are characterized and utilized in several applications, including cell transfection.

Original language	English
Pages (from-to)	65-68
Number of pages	4
Journal	Journal of Materials Chemistry
Volume	21
Issue number	1
DOIs	https://doi.org/10.1039/c0jm02819c
Publication status	Published - 7 Jan 2011

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abstract = "We report on a bioconjugation platform based on a high-affinity protein pair, barstar:barnase (Bs:Bn), that provides a modular design toolkit capable of strong (covalent) and versatile assembly of bio/nanocomplexes. Luminescent nanodiamonds (140 nm) serve as the docking station to synthesize Bs:Bn bioconjugates which are characterized and utilized in several applications, including cell transfection.",

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AU - Sreenivasan, Varun K A

AU - Ivukina, Ekaterina A.

AU - Deng, Wei

AU - Kelf, Timothy A.

AU - Zdobnova, Tatyana A.

AU - Lukash, Sergey V.

AU - Veryugin, Boris V.

AU - Stremovskiy, Oleg A.

AU - Zvyagin, Andrei V.

AU - Deyev, Sergey M.

PY - 2011/1/7

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AB - We report on a bioconjugation platform based on a high-affinity protein pair, barstar:barnase (Bs:Bn), that provides a modular design toolkit capable of strong (covalent) and versatile assembly of bio/nanocomplexes. Luminescent nanodiamonds (140 nm) serve as the docking station to synthesize Bs:Bn bioconjugates which are characterized and utilized in several applications, including cell transfection.

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Barstar:barnase - a versatile platform for colloidal diamond bioconjugation

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