Barstar:barnase - a versatile platform for colloidal diamond bioconjugation

Varun K A Sreenivasan; Ekaterina A. Ivukina; Wei Deng; Timothy A. Kelf; Tatyana A. Zdobnova; Sergey V. Lukash; Boris V. Veryugin; Oleg A. Stremovskiy; Andrei V. Zvyagin; Sergey M. Deyev

doi:10.1039/c0jm02819c

Barstar:barnase - a versatile platform for colloidal diamond bioconjugation

Varun K A Sreenivasan, Ekaterina A. Ivukina, Wei Deng, Timothy A. Kelf, Tatyana A. Zdobnova, Sergey V. Lukash, Boris V. Veryugin, Oleg A. Stremovskiy, Andrei V. Zvyagin, Sergey M. Deyev

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

We report on a bioconjugation platform based on a high-affinity protein pair, barstar:barnase (Bs:Bn), that provides a modular design toolkit capable of strong (covalent) and versatile assembly of bio/nanocomplexes. Luminescent nanodiamonds (140 nm) serve as the docking station to synthesize Bs:Bn bioconjugates which are characterized and utilized in several applications, including cell transfection.

Original language	English
Pages (from-to)	65-68
Number of pages	4
Journal	Journal of Materials Chemistry
Volume	21
Issue number	1
DOIs	https://doi.org/10.1039/c0jm02819c
Publication status	Published - 7 Jan 2011

Access to Document

10.1039/c0jm02819c

Cite this

@article{e3c99b2eebe44643ad244dee1b94f9b3,

title = "Barstar:barnase - a versatile platform for colloidal diamond bioconjugation",

abstract = "We report on a bioconjugation platform based on a high-affinity protein pair, barstar:barnase (Bs:Bn), that provides a modular design toolkit capable of strong (covalent) and versatile assembly of bio/nanocomplexes. Luminescent nanodiamonds (140 nm) serve as the docking station to synthesize Bs:Bn bioconjugates which are characterized and utilized in several applications, including cell transfection.",

author = "Sreenivasan, {Varun K A} and Ivukina, {Ekaterina A.} and Wei Deng and Kelf, {Timothy A.} and Zdobnova, {Tatyana A.} and Lukash, {Sergey V.} and Veryugin, {Boris V.} and Stremovskiy, {Oleg A.} and Zvyagin, {Andrei V.} and Deyev, {Sergey M.}",

year = "2011",

month = jan,

day = "7",

doi = "10.1039/c0jm02819c",

language = "English",

volume = "21",

pages = "65--68",

journal = "Journal of Materials Chemistry",

issn = "0959-9428",

publisher = "Royal Society of Chemistry",

number = "1",

}

TY - JOUR

T1 - Barstar:barnase - a versatile platform for colloidal diamond bioconjugation

AU - Sreenivasan, Varun K A

AU - Ivukina, Ekaterina A.

AU - Deng, Wei

AU - Kelf, Timothy A.

AU - Zdobnova, Tatyana A.

AU - Lukash, Sergey V.

AU - Veryugin, Boris V.

AU - Stremovskiy, Oleg A.

AU - Zvyagin, Andrei V.

AU - Deyev, Sergey M.

PY - 2011/1/7

Y1 - 2011/1/7

N2 - We report on a bioconjugation platform based on a high-affinity protein pair, barstar:barnase (Bs:Bn), that provides a modular design toolkit capable of strong (covalent) and versatile assembly of bio/nanocomplexes. Luminescent nanodiamonds (140 nm) serve as the docking station to synthesize Bs:Bn bioconjugates which are characterized and utilized in several applications, including cell transfection.

AB - We report on a bioconjugation platform based on a high-affinity protein pair, barstar:barnase (Bs:Bn), that provides a modular design toolkit capable of strong (covalent) and versatile assembly of bio/nanocomplexes. Luminescent nanodiamonds (140 nm) serve as the docking station to synthesize Bs:Bn bioconjugates which are characterized and utilized in several applications, including cell transfection.

UR - http://www.scopus.com/inward/record.url?scp=78649872664&partnerID=8YFLogxK

U2 - 10.1039/c0jm02819c

DO - 10.1039/c0jm02819c

M3 - Article

AN - SCOPUS:78649872664

SN - 0959-9428

VL - 21

SP - 65

EP - 68

JO - Journal of Materials Chemistry

JF - Journal of Materials Chemistry

IS - 1

ER -

Barstar:barnase - a versatile platform for colloidal diamond bioconjugation

Abstract

Access to Document

Other files and links

Fingerprint

Cite this