Bats as reservoirs of antibiotic resistance determinants: a survey of class 1 integrons in Grey-headed Flying Foxes (Pteropus poliocephalus)

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Abstract

Increasing reports of antimicrobial resistance in wildlife highlight the significance of a One Health approach to managing resistance. We investigated the prevalence and diversity of class 1 integrons, a genetic determinant of resistance, in grey-headed flying foxes, a large fruit bat species belonging to the order Chiroptera. Class 1 integrons were detected in both wild flying foxes (5.3%) and captive flying foxes (41.2%) housed in wildlife rehabilitation facilities. Genes encoding resistance to aminoglycosides, trimethoprim and beta-lactams, and Qac efflux pumps were detected. Analysis of conserved integron elements and gene cassette arrays indicate the direction of integron transfer is from humans to flying foxes. The detection of two novel gene cassette arrays (5′CS-qacH-aacA34-bla OXA-21 -3′CS and 5′CS-qacF-3′CS strongly suggests acquisition of genes from the environmental resistome into class 1 integrons within the flying fox microbiota. The dynamics of class 1 integrons in flying foxes indicates bats have a role in the emergence of novel antibiotic resistance determinants.

LanguageEnglish
Pages107-113
Number of pages7
JournalInfection, Genetics and Evolution
Volume70
DOIs
Publication statusPublished - 2019

Fingerprint

Pteropus poliocephalus
Integrons
Pteropodidae
antibiotic resistance
Microbial Drug Resistance
bat
Chiroptera
gene
genes
wildlife rehabilitation
beta-lactams
aminoglycosides
trimethoprim
pump
Genes
fruit
transporters
wildlife
Surveys and Questionnaires
Trimethoprim

Keywords

  • antimicrobial resistance
  • bats
  • integron
  • One Health
  • resistome
  • reverse zoonosis

Cite this

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title = "Bats as reservoirs of antibiotic resistance determinants: a survey of class 1 integrons in Grey-headed Flying Foxes (Pteropus poliocephalus)",
abstract = "Increasing reports of antimicrobial resistance in wildlife highlight the significance of a One Health approach to managing resistance. We investigated the prevalence and diversity of class 1 integrons, a genetic determinant of resistance, in grey-headed flying foxes, a large fruit bat species belonging to the order Chiroptera. Class 1 integrons were detected in both wild flying foxes (5.3{\%}) and captive flying foxes (41.2{\%}) housed in wildlife rehabilitation facilities. Genes encoding resistance to aminoglycosides, trimethoprim and beta-lactams, and Qac efflux pumps were detected. Analysis of conserved integron elements and gene cassette arrays indicate the direction of integron transfer is from humans to flying foxes. The detection of two novel gene cassette arrays (5′CS-qacH-aacA34-bla OXA-21 -3′CS and 5′CS-qacF-3′CS strongly suggests acquisition of genes from the environmental resistome into class 1 integrons within the flying fox microbiota. The dynamics of class 1 integrons in flying foxes indicates bats have a role in the emergence of novel antibiotic resistance determinants.",
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AB - Increasing reports of antimicrobial resistance in wildlife highlight the significance of a One Health approach to managing resistance. We investigated the prevalence and diversity of class 1 integrons, a genetic determinant of resistance, in grey-headed flying foxes, a large fruit bat species belonging to the order Chiroptera. Class 1 integrons were detected in both wild flying foxes (5.3%) and captive flying foxes (41.2%) housed in wildlife rehabilitation facilities. Genes encoding resistance to aminoglycosides, trimethoprim and beta-lactams, and Qac efflux pumps were detected. Analysis of conserved integron elements and gene cassette arrays indicate the direction of integron transfer is from humans to flying foxes. The detection of two novel gene cassette arrays (5′CS-qacH-aacA34-bla OXA-21 -3′CS and 5′CS-qacF-3′CS strongly suggests acquisition of genes from the environmental resistome into class 1 integrons within the flying fox microbiota. The dynamics of class 1 integrons in flying foxes indicates bats have a role in the emergence of novel antibiotic resistance determinants.

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