TY - JOUR
T1 - Beneficial cardiac effects of caloric restriction are lost with age in a murine model of obesity
AU - Alghatrif, Majd
AU - Watts, Vabren L.
AU - Niu, Xiaolin
AU - Halushka, Marc
AU - Miller, Karen L.
AU - Vandegaer, Konrad
AU - Bedja, Djahida
AU - Fox-Talbot, Karen
AU - Bielawska, Alicja
AU - Gabrielson, Kathleen L.
AU - Barouch, Lili A.
PY - 2013/6
Y1 - 2013/6
N2 - Obesity is associated with increased diastolic stiffness and myocardial steatosis and dysfunction. The impact of aging on the protective effects of caloric restriction (CR) is not clear. We studied 2-month (younger) and 6-7-month (older)-old ob/ob mice and age-matched C57BL/6J controls (WT). Ob/ob mice were assigned to diet ad libitum or CR for 4 weeks. We performed echocardiograms, myocardial triglyceride assays, Oil Red O staining, and measured free fatty acids, superoxide, NOS activity, ceramide levels, and Western blots. In younger mice, CR restored diastolic function, reversed myocardial steatosis, and upregulated Akt phosphorylation. None of these changes was observed in the older mice; however, CR decreased oxidative stress and normalized NOS activity in these animals. Interestingly, myocardial steatosis was not associated with increased ceramide, but CR altered the composition of ceramides. In this model of obesity, aging attenuates the benefits of CR on myocardial structure and function.
AB - Obesity is associated with increased diastolic stiffness and myocardial steatosis and dysfunction. The impact of aging on the protective effects of caloric restriction (CR) is not clear. We studied 2-month (younger) and 6-7-month (older)-old ob/ob mice and age-matched C57BL/6J controls (WT). Ob/ob mice were assigned to diet ad libitum or CR for 4 weeks. We performed echocardiograms, myocardial triglyceride assays, Oil Red O staining, and measured free fatty acids, superoxide, NOS activity, ceramide levels, and Western blots. In younger mice, CR restored diastolic function, reversed myocardial steatosis, and upregulated Akt phosphorylation. None of these changes was observed in the older mice; however, CR decreased oxidative stress and normalized NOS activity in these animals. Interestingly, myocardial steatosis was not associated with increased ceramide, but CR altered the composition of ceramides. In this model of obesity, aging attenuates the benefits of CR on myocardial structure and function.
UR - http://www.scopus.com/inward/record.url?scp=84877731498&partnerID=8YFLogxK
U2 - 10.1007/s12265-013-9453-4
DO - 10.1007/s12265-013-9453-4
M3 - Article
C2 - 23456569
AN - SCOPUS:84877731498
SN - 1937-5387
VL - 6
SP - 436
EP - 445
JO - Journal of Cardiovascular Translational Research
JF - Journal of Cardiovascular Translational Research
IS - 3
ER -