A technique that facilitates the discrimination of probe fluorescence from spurious background autofluorescence was developed. The strongly fluorescent europium chelate BHHCT and its homologous were successfully employed for time-resolved fluorescence studies. Stability of the BHHST immunoconjugates in phosphate buffered saline was determined without the addition of any stabilizing agents. The results show that threshold processed images can be analyzed by machine-vision systems to automate the analysis of samples that are difficult and tedious for human operators due to the high level of background autofluorescence.
|Number of pages||12|
|Journal||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Publication status||Published - 2005|