BHHST: an improved lanthanide chelate for time-resolved fluorescence applications

Russell Connally*, Dayong Jin, James Piper

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


A technique that facilitates the discrimination of probe fluorescence from spurious background autofluorescence was developed. The strongly fluorescent europium chelate BHHCT and its homologous were successfully employed for time-resolved fluorescence studies. Stability of the BHHST immunoconjugates in phosphate buffered saline was determined without the addition of any stabilizing agents. The results show that threshold processed images can be analyzed by machine-vision systems to automate the analysis of samples that are difficult and tedious for human operators due to the high level of background autofluorescence.

Original languageEnglish
Article number14
Pages (from-to)93-104
Number of pages12
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Publication statusPublished - 2005


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