Bi-functionalized aminoguanidine-PEGylated periodic mesoporous organosilica nanoparticles: a promising nanocarrier for delivery of Cas9-sgRNA ribonucleoproteine

Pardis Rahimi Salekdeh, Leila Ma’mani*, Javad Tavakkoly-Bazzaz, Hossein Mousavi, Mohammad Hossein Modarressi, Ghasem Hosseini Salekdeh*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

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    Abstract

    Background: There is a great interest in the efficient intracellular delivery of Cas9-sgRNA ribonucleoprotein complex (RNP) and its possible applications for in vivo CRISPR-based gene editing. In this study, a nanoporous mediated gene-editing approach has been successfully performed using a bi-functionalized aminoguanidine-PEGylated periodic mesoporous organosilica (PMO) nanoparticles (RNP@AGu@PEG1500-PMO) as a potent and biocompatible nanocarrier for RNP delivery. 

    Results: The bi-functionalized MSN-based nanomaterials have been fully characterized using electron microscopy (TEM and SEM), nitrogen adsorption measurements, thermogravimetric analysis (TGA), X-ray powder diffraction (XRD), Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR), and dynamic light scattering (DLS). The results confirm that AGu@PEG1500-PMO can be applied for gene-editing with an efficiency of about 40% as measured by GFP gene knockdown of HT1080-GFP cells with no notable change in the morphology of the cells.

    Conclusions: Due to the high stability and biocompatibility, simple synthesis, and cost-effectiveness, the developed bi-functionalized PMO-based nano-network introduces a tailored nanocarrier that has remarkable potential as a promising trajectory for biomedical and RNP delivery applications.

    Original languageEnglish
    Article number95
    Pages (from-to)1-16
    Number of pages16
    JournalJournal of Nanobiotechnology
    Volume19
    Issue number1
    DOIs
    Publication statusPublished - 31 Mar 2021

    Bibliographical note

    Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

    Keywords

    • Aminoguanidine Functionalized
    • Bi-functionalized Periodic Mesoporous Organosilica Nanoparticles
    • Cas9-sgRNA Ribonucleoproteine
    • Delivery nano-system
    • Gene editing
    • PEGylated
    • RNP

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