Bifenazate as compatible acaricide for biocontrol agent Transeius montdorensis (Acari: Phytoseiidae)

Background: Integrated pest management strategies often include the use of pesticides that have minimal impact on biological control agents. The predatory mite Transeius montdorensis is a valuable agent for controlling whiteflies, thrips and spider mites, but its tolerance to commonly used pesticides is not well understood. This study aims to investigate the genetic basis of pesticide tolerance in Transeius montdorensis. 

Results: Through genome sequencing, we examined 12 key pesticide target genes in Transeius montdorensis, including those related to acetylcholinesterase and gamma-aminobutyric acid (GABA) receptors. Our results show that Transeius montdorensis exhibits natural tolerance to bifenazate owing to the presence of the asparagine (N) residue at position 252 in cytochrome b, similar to resistance mechanisms observed in the two-spotted spider mite. In addition, we found multiple mutations in GABA receptor subunits, suggesting fipronil as a compatible pesticide for controlling thrips and whiteflies. 

Conclusion: This study provides critical genomic insights into pesticide resistance in Transeius montdorensis, advancing our understanding of its compatibility with various pesticides. These findings have significant implications for enhancing integrated pest management programmes by ensuring the effective use of Transeius montdorensis as a biological control agent without compromising its efficacy through pesticide exposure.