Bio-multifunctional noncovalent porphyrin functionalized carbon-based nanocomposite

Navid Rabiee, Mojtaba Bagherzadeh*, Amir Mohammad Ghadiri, Yousef Fatahi, Nafiseh Baheiraei, Moein Safarkhani, Abdullah Aldhaher, Rassoul Dinarvand

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)
33 Downloads (Pure)


Herein, in a one-pot method, the reduced graphene oxide layers with the assistance of multiwalled carbon nanotubes were decorated to provide a suitable space for the in situ growth of CoNi2S4, and the porphyrins were incorporated into the layers as well to increase the sensitivity of the prepared nanostructure. The prepared nanocomposite can establish π–π interactions between the genetic material and on the surface of porphyrin rings. Also, hydrogen bonds between genetic domains and the porphyrin’ nitrogen and the surface hydroxyl groups are probable. Furthermore, the potential donor–acceptor relationship between the d7 transition metal, cobalt, and the genetic material provides a suitable way to increase the interaction and gene loading , and transfections. The reason for this phenomenon was optimized to increase the EGFP by up to 17.9%. Furthermore, the sensing ability of the nanocomposite towards H2O2 was investigated. In this regard, the limit of detection of the H2O2 obtained 10 µM. Also, the in situ biosensing ability in the HEK-293 and PC12 cell lines was evaluated by the addition of PMA. The nanocomposite showed the ability to detect the released H2O2 after adding the minimum amount of 120 ng/mL of the PMA.

Original languageEnglish
Article number6604
Pages (from-to)1-15
Number of pages15
JournalScientific Reports
Publication statusPublished - 2021
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.


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