Bioactive heparin immobilized onto microfluidic channels in poly(dimethylsiloxane) results in hydrophilic surface properties

Sara Thorslund, Javier Sanchez, Rolf Larsson, Fredrik Nikolajeff, Jonas Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A new composition of heparin coating for microfluidic systems made out of poly(dimethylsiloxane) (PDMS) was developed and evaluated. The coating that consists of a conditioning polyamine layer followed by two heparin/ glutaraldehyde layers, resulted in channel surfaces with sufficient wettability to obtain flow of human normal plasma by capillary force alone. Hydrophilic channel walls are a desirable characteristic in microfluidic devices, since alternative pumping mechanisms must otherwise be included into the system. The immobilized heparin showed high antithrombin-binding capacity and a low degree of blood-material interaction. Plasma in contact with heparin-coated PDMS formed no detectable fibrin in a spectrophotometric assay by which plasma in contact with non-treated PDMS showed complete coagulation. The quartz crystal microbalance technique with energy dissipation monitoring (QCM-D) was utilized to obtain detailed information regarding adsorption kinetics and structural properties of the different layers composing the heparin coating.

Original languageEnglish
Pages (from-to)240-247
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume46
Issue number4
DOIs
Publication statusPublished - 30 Dec 2005

Keywords

  • Blood
  • Capillary flow
  • Heparin
  • Hydrophilicity
  • PDMS
  • Poly(dimethylsiloxane)
  • QCM-D

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