Bioactivity and chemical characterisation of Lophostemon suaveolens - an endemic Australian Aboriginal traditional medicinal plant

Tarannum Naz, Joanne Packer, Ping Yin, Joseph J. Brophy, Hans Wohlmuth, Dane E. Renshaw, Joshua Smith, Yaegl Community Elders, Subramanyam R. Vemulpad, Joanne F. Jamie*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    Lophostemon suaveolens is a relatively unexplored endemic medicinal plant of Australia. Extracts of fresh leaves of L. suaveolens obtained from sequential extraction with n-hexane and dichloromethane exhibited antibacterial activity in the disc diffusion and MTT microdilution assays against Streptococcus pyogenes and methicillin sensitive and resistant strains of Staphylococcus aureus (minimum bactericidal concentration < 63 g/mL). The dichloromethane extract and chromatographic fractions therein inhibited nitric oxide in RAW264.7 murine macrophages (IC50 3.7-11.6 g/mL) and also PGE2 in 3T3 murine fibroblasts (IC50 2.8-19.7 g/mL). The crude n-hexane, dichloromethane and water extracts of the leaves and chromatographic fractions from the dichloromethane extract also showed modest antioxidant activity in the ORAC assay. GC-MS analysis of the n-hexane fraction showed the presence of the antibacterial compounds aromadendrene, spathulenol, β-caryophyllene, humulene and pinene and the anti-inflammatory compounds β-caryophyllene and spathulenol. Fractionation of the dichloromethane extract led to the isolation of eucalyptin and the known anti-inflammatory compound betulinic acid.

    Original languageEnglish
    Pages (from-to)693-696
    Number of pages4
    JournalNatural Product Research
    Volume30
    Issue number6
    DOIs
    Publication statusPublished - 18 Mar 2016

    Keywords

    • apple gum
    • antimicrobial
    • Staphylococcus aureus
    • anti-inflammatory
    • antioxidant

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