Bioactivity and chemical characterisation of Lophostemon suaveolens - an endemic Australian Aboriginal traditional medicinal plant

Tarannum Naz, Joanne Packer, Ping Yin, Joseph J. Brophy, Hans Wohlmuth, Dane E. Renshaw, Joshua Smith, Yaegl Community Elders, Subramanyam R. Vemulpad, Joanne F. Jamie

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Lophostemon suaveolens is a relatively unexplored endemic medicinal plant of Australia. Extracts of fresh leaves of L. suaveolens obtained from sequential extraction with n-hexane and dichloromethane exhibited antibacterial activity in the disc diffusion and MTT microdilution assays against Streptococcus pyogenes and methicillin sensitive and resistant strains of Staphylococcus aureus (minimum bactericidal concentration < 63 g/mL). The dichloromethane extract and chromatographic fractions therein inhibited nitric oxide in RAW264.7 murine macrophages (IC50 3.7-11.6 g/mL) and also PGE2 in 3T3 murine fibroblasts (IC50 2.8-19.7 g/mL). The crude n-hexane, dichloromethane and water extracts of the leaves and chromatographic fractions from the dichloromethane extract also showed modest antioxidant activity in the ORAC assay. GC-MS analysis of the n-hexane fraction showed the presence of the antibacterial compounds aromadendrene, spathulenol, β-caryophyllene, humulene and pinene and the anti-inflammatory compounds β-caryophyllene and spathulenol. Fractionation of the dichloromethane extract led to the isolation of eucalyptin and the known anti-inflammatory compound betulinic acid.

LanguageEnglish
Pages693-696
Number of pages4
JournalNatural Product Research
Volume30
Issue number6
DOIs
Publication statusPublished - 18 Mar 2016

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Methylene Chloride
methylene chloride
Medicinal Plants
Bioactivity
medicinal plants
hexane
spathulenol
beta-caryophyllene
extracts
Inhibitory Concentration 50
inhibitory concentration 50
Assays
Anti-Inflammatory Agents
Streptococcus pyogenes
humulene
Methicillin Resistance
Methicillin
Macrophages
methicillin
mice

Keywords

  • apple gum
  • antimicrobial
  • Staphylococcus aureus
  • anti-inflammatory
  • antioxidant

Cite this

Naz, Tarannum ; Packer, Joanne ; Yin, Ping ; Brophy, Joseph J. ; Wohlmuth, Hans ; Renshaw, Dane E. ; Smith, Joshua ; Elders, Yaegl Community ; Vemulpad, Subramanyam R. ; Jamie, Joanne F. / Bioactivity and chemical characterisation of Lophostemon suaveolens - an endemic Australian Aboriginal traditional medicinal plant. In: Natural Product Research. 2016 ; Vol. 30, No. 6. pp. 693-696.
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Bioactivity and chemical characterisation of Lophostemon suaveolens - an endemic Australian Aboriginal traditional medicinal plant. / Naz, Tarannum; Packer, Joanne; Yin, Ping; Brophy, Joseph J.; Wohlmuth, Hans; Renshaw, Dane E.; Smith, Joshua; Elders, Yaegl Community; Vemulpad, Subramanyam R.; Jamie, Joanne F.

In: Natural Product Research, Vol. 30, No. 6, 18.03.2016, p. 693-696.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Naz, Tarannum

AU - Packer, Joanne

AU - Yin, Ping

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AU - Wohlmuth, Hans

AU - Renshaw, Dane E.

AU - Smith, Joshua

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AU - Jamie, Joanne F.

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