Abstract
An aerobic bacterium capable of growth on cis-dichloroethene (cDCE) as a sole carbon and energy source was isolated by enrichment culture. The 16S ribosomal DNA sequence of the isolate (strain JS666) had 97.9% identity to the sequence from Polaromonas vacuolata, indicating that the isolate was a β-proteobacterium. At 20°C, strain JS666 grew on cDCE with a minimum doubling time of 73 ± 7 h and a growth yield of 6.1 g of protein/mol of cDCE. Chloride analysis indicated that complete dechlorination of cDCE occurred during growth. The half-velocity constant for cDCE transformation was 1.6 ± 0.2 muM, and the maximum specific substrate utilization rate ranged from 12.6 to 16.8 nmol/min/mg of protein. Resting cells grown on cDCE could transform cDCE, ethene, vinyl chloride, trans-dichloroethene, trichloroethene, and 1,2-dichloroethane. Epoxyethane was produced from ethene by cDCE-grown cells, suggesting that an epoxidation reaction is the first step in cDCE degradation.
Original language | English |
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Pages (from-to) | 2726-2730 |
Number of pages | 5 |
Journal | Applied and Environmental Microbiology |
Volume | 68 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 2002 |
Externally published | Yes |
Keywords
- REDUCTIVELY DECHLORINATES TETRACHLOROETHENE
- METHYLOSINUS-TRICHOSPORIUM OB3B
- VINYL-CHLORIDE
- CHLORINATED HYDROCARBONS
- GEN. NOV.
- METABOLISM
- SUBSTRATE
- BACTERIUM
- ETHENE
- GROWTH