Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film

H. Y. Zhao; W. Zheng; Z. X. Meng; H. M. Zhou; X. X. Xu; Z. Li; Y. F. Zheng

doi:10.1016/j.bios.2008.12.004

Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film

H. Y. Zhao, W. Zheng, Z. X. Meng, H. M. Zhou, X. X. Xu, Z. Li, Y. F. Zheng^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

144 Citations (Scopus)

Abstract

This study described the bioelectrochemistry of hemoglobin (Hb) at multiwall carbon nanotubes (MWCNTs) noncovalently functionalized with biopolymers of sodium alginate (SA). The characteristics of Hb/SA-MWCNTs composite film were studied by using FT-IR spectroscopy, UV-vis spectroscopy and electrochemical methods. Hb immobilized on SA-MWCNTs composite film retained its native secondary structure, achieved direct electron transfer with the apparent heterogeneous electron transfer rate constant (k_s) of (9.54 ± 0.883) s^-1 and showed excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide. The amperometric response of the biosensor varied linearly with the H₂O₂ concentration ranging from 40 to 200 μM, with a detection limit of 16.41 × 10^-6 M (S/N = 3) and the good long-term stability. Finally, we applied this proposed method to investigate the concentration of hydrogen peroxide in real samples.

Original language	English
Pages (from-to)	2352-2357
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	24
Issue number	8
DOIs	https://doi.org/10.1016/j.bios.2008.12.004
Publication status	Published - 15 Apr 2009
Externally published	Yes

Keywords

Biosensor
Direct electron transfer
Electrocatalysis
Hemoglobin
Sodium alginate-multiwall carbon nanotubes composite film

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10.1016/j.bios.2008.12.004

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title = "Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film",

abstract = "This study described the bioelectrochemistry of hemoglobin (Hb) at multiwall carbon nanotubes (MWCNTs) noncovalently functionalized with biopolymers of sodium alginate (SA). The characteristics of Hb/SA-MWCNTs composite film were studied by using FT-IR spectroscopy, UV-vis spectroscopy and electrochemical methods. Hb immobilized on SA-MWCNTs composite film retained its native secondary structure, achieved direct electron transfer with the apparent heterogeneous electron transfer rate constant (ks) of (9.54 ± 0.883) s-1 and showed excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 40 to 200 μM, with a detection limit of 16.41 × 10-6 M (S/N = 3) and the good long-term stability. Finally, we applied this proposed method to investigate the concentration of hydrogen peroxide in real samples.",

keywords = "Biosensor, Direct electron transfer, Electrocatalysis, Hemoglobin, Sodium alginate-multiwall carbon nanotubes composite film",

author = "Zhao, {H. Y.} and W. Zheng and Meng, {Z. X.} and Zhou, {H. M.} and Xu, {X. X.} and Z. Li and Zheng, {Y. F.}",

year = "2009",

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doi = "10.1016/j.bios.2008.12.004",

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AU - Zhao, H. Y.

AU - Zheng, W.

AU - Meng, Z. X.

AU - Zhou, H. M.

AU - Xu, X. X.

AU - Li, Z.

AU - Zheng, Y. F.

PY - 2009/4/15

Y1 - 2009/4/15

N2 - This study described the bioelectrochemistry of hemoglobin (Hb) at multiwall carbon nanotubes (MWCNTs) noncovalently functionalized with biopolymers of sodium alginate (SA). The characteristics of Hb/SA-MWCNTs composite film were studied by using FT-IR spectroscopy, UV-vis spectroscopy and electrochemical methods. Hb immobilized on SA-MWCNTs composite film retained its native secondary structure, achieved direct electron transfer with the apparent heterogeneous electron transfer rate constant (ks) of (9.54 ± 0.883) s-1 and showed excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 40 to 200 μM, with a detection limit of 16.41 × 10-6 M (S/N = 3) and the good long-term stability. Finally, we applied this proposed method to investigate the concentration of hydrogen peroxide in real samples.

AB - This study described the bioelectrochemistry of hemoglobin (Hb) at multiwall carbon nanotubes (MWCNTs) noncovalently functionalized with biopolymers of sodium alginate (SA). The characteristics of Hb/SA-MWCNTs composite film were studied by using FT-IR spectroscopy, UV-vis spectroscopy and electrochemical methods. Hb immobilized on SA-MWCNTs composite film retained its native secondary structure, achieved direct electron transfer with the apparent heterogeneous electron transfer rate constant (ks) of (9.54 ± 0.883) s-1 and showed excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 40 to 200 μM, with a detection limit of 16.41 × 10-6 M (S/N = 3) and the good long-term stability. Finally, we applied this proposed method to investigate the concentration of hydrogen peroxide in real samples.

KW - Biosensor

KW - Direct electron transfer

KW - Electrocatalysis

KW - Hemoglobin

KW - Sodium alginate-multiwall carbon nanotubes composite film

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SP - 2352

EP - 2357

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 8

ER -

Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film

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Keywords

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