Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film

H. Y. Zhao, W. Zheng, Z. X. Meng, H. M. Zhou, X. X. Xu, Z. Li, Y. F. Zheng*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

136 Citations (Scopus)

Abstract

This study described the bioelectrochemistry of hemoglobin (Hb) at multiwall carbon nanotubes (MWCNTs) noncovalently functionalized with biopolymers of sodium alginate (SA). The characteristics of Hb/SA-MWCNTs composite film were studied by using FT-IR spectroscopy, UV-vis spectroscopy and electrochemical methods. Hb immobilized on SA-MWCNTs composite film retained its native secondary structure, achieved direct electron transfer with the apparent heterogeneous electron transfer rate constant (ks) of (9.54 ± 0.883) s-1 and showed excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 40 to 200 μM, with a detection limit of 16.41 × 10-6 M (S/N = 3) and the good long-term stability. Finally, we applied this proposed method to investigate the concentration of hydrogen peroxide in real samples.

Original languageEnglish
Pages (from-to)2352-2357
Number of pages6
JournalBiosensors and Bioelectronics
Volume24
Issue number8
DOIs
Publication statusPublished - 15 Apr 2009
Externally publishedYes

Keywords

  • Biosensor
  • Direct electron transfer
  • Electrocatalysis
  • Hemoglobin
  • Sodium alginate-multiwall carbon nanotubes composite film

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