Biofunctionalization of silica-coated magnetic particles mediated by a peptide

Andrew Care, Fei Chi, Peter L. Bergquist, Anwar Sunna

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A linker peptide sequence with affinity to silica-containing materials was fused to Streptococcus protein G', an antibody-binding protein. This recombinant fusion protein, linker-protein G (LPG) was produced in E. coli and exhibited strong affinity to silica-coated magnetic particles and was able to bind to them at different pHs, indicating a true pH-independent binding. LPG was used as an anchorage point for the oriented immobilization of antibodies onto the surface of the particles. These particle-bound "LPG-Antibody complexes" mediated the binding and recovery of different cell types (e.g., human stem cells, Legionella, Cryptosporidium and Giardia), enabling their rapid and simple visualization and identification. This strategy was used also for the efficient capture of Cryptosporidium oocysts from water samples. These results demonstrate that LPG can mediate the direct biofunctionalization of silica-coated magnetic particles without the need for complex surface chemical modification.

LanguageEnglish
Article number2543
Pages1-9
Number of pages9
JournalJournal of Nanoparticle Research
Volume16
Issue number8
DOIs
Publication statusPublished - 2014

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G Protein
Silica
Peptides
Silicon Dioxide
peptides
silicon dioxide
proteins
Proteins
Antibody
Antibodies
antibodies
Affine transformation
Recombinant Fusion Proteins
Chemical modification
Protein
Stem cells
affinity
Stem Cells
Escherichia coli
Carrier Proteins

Cite this

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abstract = "A linker peptide sequence with affinity to silica-containing materials was fused to Streptococcus protein G', an antibody-binding protein. This recombinant fusion protein, linker-protein G (LPG) was produced in E. coli and exhibited strong affinity to silica-coated magnetic particles and was able to bind to them at different pHs, indicating a true pH-independent binding. LPG was used as an anchorage point for the oriented immobilization of antibodies onto the surface of the particles. These particle-bound {"}LPG-Antibody complexes{"} mediated the binding and recovery of different cell types (e.g., human stem cells, Legionella, Cryptosporidium and Giardia), enabling their rapid and simple visualization and identification. This strategy was used also for the efficient capture of Cryptosporidium oocysts from water samples. These results demonstrate that LPG can mediate the direct biofunctionalization of silica-coated magnetic particles without the need for complex surface chemical modification.",
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Biofunctionalization of silica-coated magnetic particles mediated by a peptide. / Care, Andrew; Chi, Fei; Bergquist, Peter L.; Sunna, Anwar.

In: Journal of Nanoparticle Research, Vol. 16, No. 8, 2543, 2014, p. 1-9.

Research output: Contribution to journalArticleResearchpeer-review

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