Biogenesis of mitochondria. Phospholipid synthesis in vitro by yeast mitochondrial and microsomal fractions

G. S. Cobon, P. D. Crowfoot, A. W. Linnane

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The ability in vitro of yeast mitochondrial and microsomal fractions to synthesize lipid de novo was measured. The major phospholipids synthesized from sn-[2-3H]glycerol 3-phosphate by the two microsomal fractions were phosphatidylserine, phosphatidylinositol and phosphatidic acid. The mitochondrial fraction, which had a higher specific activity for total glycerolipid synthesis, synthesized phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and phosphatidic acid, together with smaller amounts of neutral lipids and diphosphatidylglycerol. Phosphatidylcholine synthesis from both S-adenosyl[Me-14C]methionine and CDP-[Me-14C]choline appeared to be localized in the microsomal fraction.
Original languageEnglish
Pages (from-to)265-275
Number of pages11
JournalBiochemical Journal
Volume144
Issue number2
DOIs
Publication statusPublished - 15 Nov 1974
Externally publishedYes

Fingerprint Dive into the research topics of 'Biogenesis of mitochondria. Phospholipid synthesis in vitro by yeast mitochondrial and microsomal fractions'. Together they form a unique fingerprint.

  • Cite this