Biogenic amine modulation of honey bee sociability and nestmate affiliation

Susie E. Hewlett, Jacqueline D. Delahunt Smoleniec, Deborah M. Wareham, Thomas M. Pyne, Andrew B. Barron

Research output: Contribution to journalArticle

Abstract

Biogenic amines modulate a range of social behaviours, including sociability and mechanisms of group cohesion, in both vertebrates and invertebrates. Here, we tested if the biogenic amines modulate honey bee (Apis mellifera) sociability and nestmate affiliation. We examined the consequences of treatments with biogenic amines, agonists and antagonists on a bee’s approach to, and subsequent social interactions with, conspecifics in both naturally hive-reared bees and isolated bees. We used two different treatment methods. Bees were first treated topically with compounds dissolved in the solvent dimethylformamide (dMF) applied to the dorsal thorax, but dMF had a significant effect on the locomotion and behaviour of the bees during the behavioural test that interfered with their social responses. Our second method used microinjection to deliver biogenic amines to the head capsule via the ocellar tract. Microinjection of dopamine and a dopamine antagonist had strong effects on bee sociability, likelihood of interaction with bees, and nestmate affiliation. Octopamine treatment reduced social interaction with other bees, and serotonin increased the likelihood of social interactions. HPLC measurements showed that isolation reduced brain levels of biogenic amines compared to hive-reared bees. Our findings suggest that dopamine is an important neurochemical component of social motivation in bees. This finding advances a comparative understanding of the processes of social evolution.

LanguageEnglish
Article numbere0205686
Pages1-18
Number of pages18
JournalPLoS ONE
Volume13
Issue number10
DOIs
StatePublished - 25 Oct 2018

Fingerprint

Biogenic Amines
Honey
Bees
biogenic amines
honey bees
Apoidea
Modulation
Dimethylformamide
dopamine
Dopamine
dimethylformamide
beehives
Octopamine
Interpersonal Relations
Dopamine Antagonists
antagonists
Urticaria
Microinjections
Capsules
Brain

Bibliographical note

Copyright the Author(s) 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Cite this

Hewlett, S. E., Delahunt Smoleniec, J. D., Wareham, D. M., Pyne, T. M., & Barron, A. B. (2018). Biogenic amine modulation of honey bee sociability and nestmate affiliation. PLoS ONE, 13(10), 1-18. [e0205686]. DOI: 10.1371/journal.pone.0205686
Hewlett, Susie E. ; Delahunt Smoleniec, Jacqueline D. ; Wareham, Deborah M. ; Pyne, Thomas M. ; Barron, Andrew B./ Biogenic amine modulation of honey bee sociability and nestmate affiliation. In: PLoS ONE. 2018 ; Vol. 13, No. 10. pp. 1-18
@article{378e8bcf74a144979b8892d999b76d20,
title = "Biogenic amine modulation of honey bee sociability and nestmate affiliation",
abstract = "Biogenic amines modulate a range of social behaviours, including sociability and mechanisms of group cohesion, in both vertebrates and invertebrates. Here, we tested if the biogenic amines modulate honey bee (Apis mellifera) sociability and nestmate affiliation. We examined the consequences of treatments with biogenic amines, agonists and antagonists on a bee’s approach to, and subsequent social interactions with, conspecifics in both naturally hive-reared bees and isolated bees. We used two different treatment methods. Bees were first treated topically with compounds dissolved in the solvent dimethylformamide (dMF) applied to the dorsal thorax, but dMF had a significant effect on the locomotion and behaviour of the bees during the behavioural test that interfered with their social responses. Our second method used microinjection to deliver biogenic amines to the head capsule via the ocellar tract. Microinjection of dopamine and a dopamine antagonist had strong effects on bee sociability, likelihood of interaction with bees, and nestmate affiliation. Octopamine treatment reduced social interaction with other bees, and serotonin increased the likelihood of social interactions. HPLC measurements showed that isolation reduced brain levels of biogenic amines compared to hive-reared bees. Our findings suggest that dopamine is an important neurochemical component of social motivation in bees. This finding advances a comparative understanding of the processes of social evolution.",
author = "Hewlett, {Susie E.} and {Delahunt Smoleniec}, {Jacqueline D.} and Wareham, {Deborah M.} and Pyne, {Thomas M.} and Barron, {Andrew B.}",
note = "Copyright the Author(s) 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",
year = "2018",
month = "10",
day = "25",
doi = "10.1371/journal.pone.0205686",
language = "English",
volume = "13",
pages = "1--18",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

Hewlett, SE, Delahunt Smoleniec, JD, Wareham, DM, Pyne, TM & Barron, AB 2018, 'Biogenic amine modulation of honey bee sociability and nestmate affiliation' PLoS ONE, vol 13, no. 10, e0205686, pp. 1-18. DOI: 10.1371/journal.pone.0205686

Biogenic amine modulation of honey bee sociability and nestmate affiliation. / Hewlett, Susie E.; Delahunt Smoleniec, Jacqueline D.; Wareham, Deborah M.; Pyne, Thomas M.; Barron, Andrew B.

In: PLoS ONE, Vol. 13, No. 10, e0205686, 25.10.2018, p. 1-18.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biogenic amine modulation of honey bee sociability and nestmate affiliation

AU - Hewlett,Susie E.

AU - Delahunt Smoleniec,Jacqueline D.

AU - Wareham,Deborah M.

AU - Pyne,Thomas M.

AU - Barron,Andrew B.

N1 - Copyright the Author(s) 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2018/10/25

Y1 - 2018/10/25

N2 - Biogenic amines modulate a range of social behaviours, including sociability and mechanisms of group cohesion, in both vertebrates and invertebrates. Here, we tested if the biogenic amines modulate honey bee (Apis mellifera) sociability and nestmate affiliation. We examined the consequences of treatments with biogenic amines, agonists and antagonists on a bee’s approach to, and subsequent social interactions with, conspecifics in both naturally hive-reared bees and isolated bees. We used two different treatment methods. Bees were first treated topically with compounds dissolved in the solvent dimethylformamide (dMF) applied to the dorsal thorax, but dMF had a significant effect on the locomotion and behaviour of the bees during the behavioural test that interfered with their social responses. Our second method used microinjection to deliver biogenic amines to the head capsule via the ocellar tract. Microinjection of dopamine and a dopamine antagonist had strong effects on bee sociability, likelihood of interaction with bees, and nestmate affiliation. Octopamine treatment reduced social interaction with other bees, and serotonin increased the likelihood of social interactions. HPLC measurements showed that isolation reduced brain levels of biogenic amines compared to hive-reared bees. Our findings suggest that dopamine is an important neurochemical component of social motivation in bees. This finding advances a comparative understanding of the processes of social evolution.

AB - Biogenic amines modulate a range of social behaviours, including sociability and mechanisms of group cohesion, in both vertebrates and invertebrates. Here, we tested if the biogenic amines modulate honey bee (Apis mellifera) sociability and nestmate affiliation. We examined the consequences of treatments with biogenic amines, agonists and antagonists on a bee’s approach to, and subsequent social interactions with, conspecifics in both naturally hive-reared bees and isolated bees. We used two different treatment methods. Bees were first treated topically with compounds dissolved in the solvent dimethylformamide (dMF) applied to the dorsal thorax, but dMF had a significant effect on the locomotion and behaviour of the bees during the behavioural test that interfered with their social responses. Our second method used microinjection to deliver biogenic amines to the head capsule via the ocellar tract. Microinjection of dopamine and a dopamine antagonist had strong effects on bee sociability, likelihood of interaction with bees, and nestmate affiliation. Octopamine treatment reduced social interaction with other bees, and serotonin increased the likelihood of social interactions. HPLC measurements showed that isolation reduced brain levels of biogenic amines compared to hive-reared bees. Our findings suggest that dopamine is an important neurochemical component of social motivation in bees. This finding advances a comparative understanding of the processes of social evolution.

UR - http://www.scopus.com/inward/record.url?scp=85055504862&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0205686

DO - 10.1371/journal.pone.0205686

M3 - Article

VL - 13

SP - 1

EP - 18

JO - PLoS ONE

T2 - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 10

M1 - e0205686

ER -

Hewlett SE, Delahunt Smoleniec JD, Wareham DM, Pyne TM, Barron AB. Biogenic amine modulation of honey bee sociability and nestmate affiliation. PLoS ONE. 2018 Oct 25;13(10):1-18. e0205686. Available from, DOI: 10.1371/journal.pone.0205686