Biolog phenotype microarrays for phenotypic characterization of microbial cells

Amanda M. Mackie, Karl A. Hassan, Ian T. Paulsen, Sasha G. Tetu

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

41 Citations (Scopus)

Abstract

Biolog Phenotype MicroArrays for microorganisms provide a high-throughput method for the global analysis of microbial growth phenotypes. Using a colorimetric reaction that is indicative of respiration, these microplate assays measure the response of an individual strain or microbial community to a large and diverse range of nutrients and chemicals. Phenotype MicroArrays have been used to study gene function and to improve genome annotation in single microorganisms and for physiological profiling of bacterial communities. The microplate system can be used to obtain a comprehensive overview of metabolic capability, or it can be tailored, through the use of subsets of plates, to address specific research needs.

Original languageEnglish
Title of host publicationEnvironmental microbiology
Subtitle of host publicationMethods and protocols
EditorsIan T. Paulsen, Andrew J. Holmes
Place of PublicationTotowa, NJ
PublisherHumana Press
Pages123-130
Number of pages8
Edition2nd
ISBN (Electronic)9781627037129
ISBN (Print)9781627037112
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in molecular biology
Volume1096
ISSN (Print)1064-3745

Keywords

  • Biolog
  • Metabolic fingerprinting
  • Microplate assays
  • Phenotype
  • Susceptibility testing

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