TY - JOUR
T1 - Biological and molecular variability of Zucchini yellow mosaic virus in Iran
AU - Bananej, Kaveh
AU - Keshavarz, Tayyebeh
AU - Vahdat, Aisan
AU - Hosseini Salekdeh, Ghasem
AU - Glasa, Miroslav
PY - 2008/12
Y1 - 2008/12
N2 - Zucchini yellow mosaic virus (ZYMV; family Potyviridae, genus Potyvirus) causes high yield losses to cucurbits in many parts of the world. The virus was detected for the first time in Iran in 1988, but the isolates have not been characterized. To study the genetic and biological diversity among Iranian ZYMV isolates, a set of twelve isolates, obtained during an extensive survey conducted from 2003 to 2006 in the major cucurbit-growing areas, were characterized. An experimental host range study of these isolates (referred as Iran-1 to Iran-12) revealed some variation in their biological properties. The nucleotide sequences of the genomic portion spanning the C-terminal part of NIb and N-terminal part of coat protein (CP) coding region were determined and compared with other available sequences. The identity among Iranian ZYMV isolates at the amino acid level reached 95.6-100%. The Iranian ZYMV isolates did not form a compact cluster in the phylogenetic tree, and the phylogenetic analyses and the estimation of genetic distance indicate that the Iranian ZYMV group consists of several independent introductions that evolved separately.
AB - Zucchini yellow mosaic virus (ZYMV; family Potyviridae, genus Potyvirus) causes high yield losses to cucurbits in many parts of the world. The virus was detected for the first time in Iran in 1988, but the isolates have not been characterized. To study the genetic and biological diversity among Iranian ZYMV isolates, a set of twelve isolates, obtained during an extensive survey conducted from 2003 to 2006 in the major cucurbit-growing areas, were characterized. An experimental host range study of these isolates (referred as Iran-1 to Iran-12) revealed some variation in their biological properties. The nucleotide sequences of the genomic portion spanning the C-terminal part of NIb and N-terminal part of coat protein (CP) coding region were determined and compared with other available sequences. The identity among Iranian ZYMV isolates at the amino acid level reached 95.6-100%. The Iranian ZYMV isolates did not form a compact cluster in the phylogenetic tree, and the phylogenetic analyses and the estimation of genetic distance indicate that the Iranian ZYMV group consists of several independent introductions that evolved separately.
KW - Zucchini yellow mosaic virus
KW - Potyvirus
KW - sequence analyses
KW - Iran
UR - http://www.scopus.com/inward/record.url?scp=55949123355&partnerID=8YFLogxK
U2 - 10.1111/j.1439-0434.2008.01430.x
DO - 10.1111/j.1439-0434.2008.01430.x
M3 - Article
AN - SCOPUS:55949123355
SN - 0931-1785
VL - 156
SP - 654
EP - 659
JO - Journal of Phytopathology
JF - Journal of Phytopathology
IS - 11-12
ER -