Biomacromolecules in bivalve shells with crossed lamellar architecture

Oluwatoosin B. A. Agbaje, Denise E. Thomas, J. Gabriel Dominguez, Bernie V. Mclnerney, Matthew A. Kosnik, Dorrit E. Jacob

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We present an in-depth characterisation of shells from two bivalve species with crossed lamellar microstructure, namely Tridacna gigas and Fulvia tenuicostata. High-resolution scanning electron microscopy and confocal microscopy imaging reveal a fine structure of nanogranular particles that are inorganic–bioorganic nanocomposites for both shells. In F. tenuicostata, inorganic–organic components are arranged in a polycrystalline fibre-like fabric. T. gigas consists of up to four hierarchical lamellar structural orders and the second-order lamellae consist of elongated nanometre-sized laths. The inorganic matrix is intimately intergrown with the total amount of organic matter (1.8 and 1.5 wt%), and the composition of the shell macromolecules is variable between the two calcareous biominerals. This work shows for the first time the presence of polysaccharide-based compounds that could be essential for the construction of bio-organics as well as many prominent protein bands, glycoproteins and/or glycosaminoglycans of unknown sizes far above 260 kDa in bivalve shells with crossed lamellar microstructure. Chitosan (deacetylated chitin) with apparent molecular weights from 18 to 110 kDa for T. gigas and from 12 kDa till above 110 kDa for F. tenuicostata are detected in gel electrophoresis after Calcofluor staining. In each of the shell extracts, the infrared spectroscopy shows polysaccharides, proteins and lipids. Our findings from two crossed lamellar shells representing two genera of Mollusca: Cardiidae indicate that chitin–protein complexes and lipid–lipoproteins are not restricted only to bivalves with nacroprismatic shells.

LanguageEnglish
Pages4952-4969
Number of pages18
JournalJournal of Materials Science
Volume54
Issue number6
DOIs
Publication statusPublished - Mar 2019

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Chitin
Polysaccharides
Proteins
Lipids
Lipoproteins
Glycoproteins
Microstructure
Confocal microscopy
High resolution electron microscopy
Chitosan
Glycosaminoglycans
Electrophoresis
Macromolecules
Biological materials
Infrared spectroscopy
Nanocomposites
Gels
Molecular weight
Imaging techniques
Scanning electron microscopy

Keywords

  • CLAM TRIDACNA-GIGAS
  • ORGANIC MATRIX
  • MECHANICAL-PROPERTIES
  • CHITIN DEACETYLASE
  • ACIDIC PROTEINS
  • PINNA-NOBILIS
  • STAINS-ALL
  • PART I
  • MOLLUSK
  • LAYERS

Cite this

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title = "Biomacromolecules in bivalve shells with crossed lamellar architecture",
abstract = "We present an in-depth characterisation of shells from two bivalve species with crossed lamellar microstructure, namely Tridacna gigas and Fulvia tenuicostata. High-resolution scanning electron microscopy and confocal microscopy imaging reveal a fine structure of nanogranular particles that are inorganic–bioorganic nanocomposites for both shells. In F. tenuicostata, inorganic–organic components are arranged in a polycrystalline fibre-like fabric. T. gigas consists of up to four hierarchical lamellar structural orders and the second-order lamellae consist of elongated nanometre-sized laths. The inorganic matrix is intimately intergrown with the total amount of organic matter (1.8 and 1.5 wt{\%}), and the composition of the shell macromolecules is variable between the two calcareous biominerals. This work shows for the first time the presence of polysaccharide-based compounds that could be essential for the construction of bio-organics as well as many prominent protein bands, glycoproteins and/or glycosaminoglycans of unknown sizes far above 260 kDa in bivalve shells with crossed lamellar microstructure. Chitosan (deacetylated chitin) with apparent molecular weights from 18 to 110 kDa for T. gigas and from 12 kDa till above 110 kDa for F. tenuicostata are detected in gel electrophoresis after Calcofluor staining. In each of the shell extracts, the infrared spectroscopy shows polysaccharides, proteins and lipids. Our findings from two crossed lamellar shells representing two genera of Mollusca: Cardiidae indicate that chitin–protein complexes and lipid–lipoproteins are not restricted only to bivalves with nacroprismatic shells.",
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author = "Agbaje, {Oluwatoosin B. A.} and Thomas, {Denise E.} and Dominguez, {J. Gabriel} and Mclnerney, {Bernie V.} and Kosnik, {Matthew A.} and Jacob, {Dorrit E.}",
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Biomacromolecules in bivalve shells with crossed lamellar architecture. / Agbaje, Oluwatoosin B. A.; Thomas, Denise E.; Dominguez, J. Gabriel; Mclnerney, Bernie V.; Kosnik, Matthew A.; Jacob, Dorrit E.

In: Journal of Materials Science, Vol. 54, No. 6, 03.2019, p. 4952-4969.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Biomacromolecules in bivalve shells with crossed lamellar architecture

AU - Agbaje, Oluwatoosin B. A.

AU - Thomas, Denise E.

AU - Dominguez, J. Gabriel

AU - Mclnerney, Bernie V.

AU - Kosnik, Matthew A.

AU - Jacob, Dorrit E.

PY - 2019/3

Y1 - 2019/3

N2 - We present an in-depth characterisation of shells from two bivalve species with crossed lamellar microstructure, namely Tridacna gigas and Fulvia tenuicostata. High-resolution scanning electron microscopy and confocal microscopy imaging reveal a fine structure of nanogranular particles that are inorganic–bioorganic nanocomposites for both shells. In F. tenuicostata, inorganic–organic components are arranged in a polycrystalline fibre-like fabric. T. gigas consists of up to four hierarchical lamellar structural orders and the second-order lamellae consist of elongated nanometre-sized laths. The inorganic matrix is intimately intergrown with the total amount of organic matter (1.8 and 1.5 wt%), and the composition of the shell macromolecules is variable between the two calcareous biominerals. This work shows for the first time the presence of polysaccharide-based compounds that could be essential for the construction of bio-organics as well as many prominent protein bands, glycoproteins and/or glycosaminoglycans of unknown sizes far above 260 kDa in bivalve shells with crossed lamellar microstructure. Chitosan (deacetylated chitin) with apparent molecular weights from 18 to 110 kDa for T. gigas and from 12 kDa till above 110 kDa for F. tenuicostata are detected in gel electrophoresis after Calcofluor staining. In each of the shell extracts, the infrared spectroscopy shows polysaccharides, proteins and lipids. Our findings from two crossed lamellar shells representing two genera of Mollusca: Cardiidae indicate that chitin–protein complexes and lipid–lipoproteins are not restricted only to bivalves with nacroprismatic shells.

AB - We present an in-depth characterisation of shells from two bivalve species with crossed lamellar microstructure, namely Tridacna gigas and Fulvia tenuicostata. High-resolution scanning electron microscopy and confocal microscopy imaging reveal a fine structure of nanogranular particles that are inorganic–bioorganic nanocomposites for both shells. In F. tenuicostata, inorganic–organic components are arranged in a polycrystalline fibre-like fabric. T. gigas consists of up to four hierarchical lamellar structural orders and the second-order lamellae consist of elongated nanometre-sized laths. The inorganic matrix is intimately intergrown with the total amount of organic matter (1.8 and 1.5 wt%), and the composition of the shell macromolecules is variable between the two calcareous biominerals. This work shows for the first time the presence of polysaccharide-based compounds that could be essential for the construction of bio-organics as well as many prominent protein bands, glycoproteins and/or glycosaminoglycans of unknown sizes far above 260 kDa in bivalve shells with crossed lamellar microstructure. Chitosan (deacetylated chitin) with apparent molecular weights from 18 to 110 kDa for T. gigas and from 12 kDa till above 110 kDa for F. tenuicostata are detected in gel electrophoresis after Calcofluor staining. In each of the shell extracts, the infrared spectroscopy shows polysaccharides, proteins and lipids. Our findings from two crossed lamellar shells representing two genera of Mollusca: Cardiidae indicate that chitin–protein complexes and lipid–lipoproteins are not restricted only to bivalves with nacroprismatic shells.

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KW - ORGANIC MATRIX

KW - MECHANICAL-PROPERTIES

KW - CHITIN DEACETYLASE

KW - ACIDIC PROTEINS

KW - PINNA-NOBILIS

KW - STAINS-ALL

KW - PART I

KW - MOLLUSK

KW - LAYERS

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U2 - 10.1007/s10853-018-3165-8

DO - 10.1007/s10853-018-3165-8

M3 - Article

VL - 54

SP - 4952

EP - 4969

JO - Journal of Materials Science

T2 - Journal of Materials Science

JF - Journal of Materials Science

SN - 0022-2461

IS - 6

ER -