Biomarker responses in zebrafish (Danio rerio) larvae exposed to pristine low-density polyethylene fragments

Ali Karami, David B. Groman, Scott P. Wilson, Patimah Ismail, Vasantha K. Neela

Research output: Contribution to journalArticleResearchpeer-review

Abstract

There are serious concerns over the adverse impacts of microplastics (MPs) on living organisms. The main objective of this study was to test the effects of MPs on the total length, weight, condition factor (CF), transcriptional level of antioxidant, anti and pro-apoptotic, and neurotransmitter genes, and the histopathology of the gill, liver, brain, kidney, and intestine in the larvae of zebrafish (Danio rerio). Fish were exposed to one of three levels of pristine low-density polyethylene (LDPE) fragments (5, 50, or 500 μg/L) for 10 or 20 days. No significant changes were observed in any of the selected biomarkers across MP concentrations at days 10 or 20. The expression of casp9 (caspase 9, apoptosis-related cysteine protease), casp3a (caspase 3, apoptosis-related cysteine protease a) and cat (catalase), however, were significantly lower in the larvae sampled at day 20 than day 10. We provide evidence that virgin short-term exposure to LDPE fragments has minimal impact on biomarker responses in D. rerio larvae.

LanguageEnglish
Pages466-475
Number of pages10
JournalEnvironmental Pollution
Volume223
DOIs
Publication statusPublished - 1 Apr 2017

Fingerprint

Cysteine Proteases
Low density polyethylenes
Polyethylene
Biomarkers
Zebrafish
Cell death
Larva
Apoptosis
Caspase 9
Antioxidants
Caspase 3
Liver
Catalase
Fish
Neurotransmitter Agents
Brain
Genes
Intestines
Fishes
Cats

Keywords

  • polyethylene
  • fragment
  • histopathology
  • antioxidant
  • apoptosis
  • neurotransmitter

Cite this

Karami, Ali ; Groman, David B. ; Wilson, Scott P. ; Ismail, Patimah ; Neela, Vasantha K. / Biomarker responses in zebrafish (Danio rerio) larvae exposed to pristine low-density polyethylene fragments. In: Environmental Pollution. 2017 ; Vol. 223. pp. 466-475.
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Biomarker responses in zebrafish (Danio rerio) larvae exposed to pristine low-density polyethylene fragments. / Karami, Ali; Groman, David B.; Wilson, Scott P.; Ismail, Patimah; Neela, Vasantha K.

In: Environmental Pollution, Vol. 223, 01.04.2017, p. 466-475.

Research output: Contribution to journalArticleResearchpeer-review

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