Interdigital or interdigitated electrodes have been extensively employed as a fundament to create various types of sensors for quite some time. The significant benefits of this type of sensors such as high sensitivity, ease of fabrication, reusability etc., have led to their applications in a variety of sectors in the field of science and technology. Biomedical application of interdigital sensors is one of the most prominent applications of them, which has recently attracted so much attention among researchers. In biomedical sector, sensors have been connected to different types of signal-conditioning circuits to process and transfer data to the monitoring part of the system. Based on the application for which interdigital sensors have been used, design, processing material, configuration and size of the sensors have been largely varied. These parameters vary the performance of the sensors in terms of efficiency and sensitivity. This chapter studies different types of interdigital sensors developed for biomedical applications and some of their challenges.