Biosynthesis of natural and halogenated plant monoterpene indole alkaloids in yeast

Samuel A. Bradley, Beata J. Lehka, Frederik G. Hansson, Khem B. Adhikari, Daniela Rago, Paulina Rubaszka, Ahmad K. Haidar, Ling Chen, Lea G. Hansen, Olga Gudich, Konstantina Giannakou, Bettina Lengger, Ryan T. Gill, Yoko Nakamura, Thomas Dugé de Bernonville, Konstantinos Koudounas, David Romero-Suarez, Ling Ding, Yijun Qiao, Thomas M. FrimurerAnja A. Petersen, Sébastien Besseau, Sandeep Kumar, Nicolas Gautron, Celine Melin, Jillian Marc, Remi Jeanneau, Sarah E. O’Connor, Vincent Courdavault, Jay D. Keasling, Jie Zhang*, Michael K. Jensen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)
7 Downloads (Pure)

Abstract

Monoterpenoid indole alkaloids (MIAs) represent a large class of plant natural products with marketed pharmaceutical activities against a wide range of indications, including cancer, malaria and hypertension. Halogenated MIAs have shown improved pharmaceutical properties; however, synthesis of new-to-nature halogenated MIAs remains a challenge. Here we demonstrate a platform for de novo biosynthesis of two MIAs, serpentine and alstonine, in baker’s yeast Saccharomyces cerevisiae and deploy it to systematically explore the biocatalytic potential of refactored MIA pathways for the production of halogenated MIAs. From this, we demonstrate conversion of individual haloindole derivatives to a total of 19 different new-to-nature haloserpentine and haloalstonine analogs. Furthermore, by process optimization and heterologous expression of a modified halogenase in the microbial MIA platform, we document de novo halogenation and biosynthesis of chloroalstonine. Together, this study highlights a microbial platform for enzymatic exploration and production of complex natural and new-to-nature MIAs with therapeutic potential. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1551-1560
Number of pages26
JournalNature Chemical Biology
Volume19
Issue number12
Early online date6 Nov 2023
DOIs
Publication statusPublished - Dec 2023
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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