Blood oxygen saturation of frozen tissue determined by hyper spectral imaging

Boy Braaf*, Annemarie Nadort, Dirk Faber, Rene Ter Wee, Ton Van Leeuwen, Maurice Aalders

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

A method is proposed for determining blood oxygen saturation in frozen tissue. The method is based on a spectral camera system equipped with an Acoustic-Optical-Tuneable-Filter. The HSI-setup is validated by measuring series of unfrozen and frozen samples of a hemoglobin-solution, a hemoglobin- intralipid mixture and whole blood with varying oxygen saturation. The theoretically predicted linear relation between oxygen saturation and absorbance was observed in both the frozen sample series and the unfrozen series. In a final proof of principal, frozen myocardial tissue was measured. Higher saturation values were recorded for ventricle and atria tissue compared to the septum and connective tissue. These results are not validated by measurements with another method. The formation of methemoglobin during freezing and the presence of myoglobin in the tissue turned out to be possible sources of error.

Original languageEnglish
Title of host publicationImaging, manipulation, and analysis of biomolecules, cells, and tissues VI
EditorsDaniel L. Farkas, Dan V. Nicolau, Robert C. Leif
Place of PublicationBellingham, Washington
PublisherSPIE
Pages685907-1-685907-10
Number of pages10
ISBN (Print)9780819470348
DOIs
Publication statusPublished - 2008
Externally publishedYes
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI - San Jose, CA, United States
Duration: 21 Jan 200823 Jan 2008

Publication series

NameProgress in biomedical optics and imaging
PublisherSPIE
Number18
Volume9
NameProceedings of SPIE
PublisherSPIE
Volume6859
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
CountryUnited States
CitySan Jose, CA
Period21/01/0823/01/08

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