Blood protein purification and simultaneous removal of nonenveloped viruses using tangential-flow preparative electrophoresis

Michael Evtushenko*, Kailing Wang, Hatch W. Stokes, Hari Nair

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Gradiflow is new technology allowing purification of important blood proteins from viral contaminated plasma. Protein purification is based on unique scalable tangential-flow preparative electrophoresis, and is distinct from current technology because protein purification and virus removal are performed in the same step. This one-step removal and purification exploits both the size and charge of target proteins. The medically important blood proteins, immunoglobulin G (IgG) and α-1-antitrypsin, were chosen to demonstrate the ability of this process to purify proteins from contaminated plasma. Clearance factors achieved by infectivity assays and polymerase chain reaction (PCR) that meet regulatory requirements demonstrated removal of canine parvovirus (CPV). CPV is a model virus for pathogenic nonenveloped viruses, including parvovirus B19, not adequately removed or inactivated by most processes currently in practice. The recovery of proteins from plasma with high purity, recovery, and function, while simultaneously removing viruses, provides blood products with a level of purity compatible with clinical use more quickly and cheaply than available techniques.

Original languageEnglish
Pages (from-to)28-34
Number of pages7
JournalElectrophoresis
Volume26
Issue number1
DOIs
Publication statusPublished - Jan 2005

Keywords

  • α-1-antitrypsin
  • Immunoglobulin G
  • Nonenveloped viruses
  • Parvovirus
  • Preparative electrophoresis

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