Blueprints for biosensors: design, limitations, and applications

    Research output: Contribution to journalArticlepeer-review

    84 Citations (Scopus)
    39 Downloads (Pure)


    Biosensors are enabling major advances in the field of analytics that are both facilitating and being facilitated by advances in synthetic biology. The ability of biosensors to rapidly and specifically detect a wide range of molecules makes them highly relevant to a range of industrial, medical, ecological, and scientific applications. Approaches to biosensor design are as diverse as their applications, with major biosensor classes including nucleic acids, proteins, and transcription factors. Each of these biosensor types has advantages and limitations based on the intended application, and the parameters that are required for optimal performance. Specifically, the choice of biosensor design must consider factors such as the ligand specificity, sensitivity, dynamic range, functional range, mode of output, time of activation, ease of use, and ease of engineering. This review discusses the rationale for designing the major classes of biosensor in the context of their limitations and assesses their suitability to different areas of biotechnological application.
    Original languageEnglish
    Article number375
    Pages (from-to)1-28
    Number of pages28
    Issue number8
    Publication statusPublished - Aug 2018

    Bibliographical note

    Copyright the Author(s) 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.


    • biosensors
    • synthetic biology
    • analytics
    • molecular diagnostics
    • protein switches
    • aptamers
    • high-throughput screening
    • metabolic engineering


    Dive into the research topics of 'Blueprints for biosensors: design, limitations, and applications'. Together they form a unique fingerprint.

    Cite this